Investigation on the absorption of phillyrin and forsythiaside in rat digestive tract

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• 作者：Yun-xia Li (1)
  Cheng Peng (1)
  Liang-hong Ye (1)
  Ruo-qi Zhang (2)
  Xue-hua Jiang (2)
  
• 关键词：Phillyrin ; Forsythiaside ; Absorption ; In situ rat model
• 刊名：European Journal of Drug Metabolism and Pharmacokinetics
• 出版年：2011
• 出版时间：June 2011
• 年：2011
• 卷：36
• 期：2
• 页码：79-85
• 全文大小：214KB
• 参考文献：1. Barr WH, Riegelman S (1970) Intestinal drug absorption and metabolism I: comparison of methods and models to study physiological factors of in vitro and in vivo intestinal absorption. J Pharm Sci 59:154-63. doi:10.1002/jps.2600590204 CrossRef
  2. Barthe L, Woodley J, Houin G (1999) Gastrointestinal absorption of drugs: methods and studies. Fundam Clin Pharmacol 13:154-68. doi:10.1111/j.1472-8206.1999.tb00334.x CrossRef
  3. Bing Q, Wei Q, Xing H et al (1999) Study on the anti-inflammatory and analgesia function of fructus forsythia extraction. Zhong Cao Yao 30:43-5. doi:cnki:sun:zcyo.01999-01-020
  4. Cheng KC, Li C (2008) Prediction of oral drug absorption in humans—from cultured cell lines and experimental animals. Expert Opin Drug Metab Toxicol 4:581-90. doi:10.1517/17425255.4.5.581 CrossRef
  5. Cui YY, Feng SY, Zhao G et al (1992) HPLC analysis of the active ingredients of Forsythia suspense. Yao Xue Xue Bao 27:603-08. doi:cnki:issn:0513-4870.0.1992-08-008
  6. Fari?a JB, Evora M, Espin D et al (1988) Theoretical model for interpretation of in situ absorption studies. Int J Pharma 45:123-28. doi:10.1016/0378-5173(88)90040-3 CrossRef
  7. Ishizuka O, Kumazawa N, Ohta S et al (1992) Effects of various methanol extracts of crude drugs on experimental subacute and chronic hepatic injury. Yakugaku Zasshi 112:174-82
  8. Kumazawa N, Ohta S, Ishizuka O et al (1990) Protective effects of various methanol extracts of crude drugs on experimental hepatic injury induced by carbon tetrachloride in rats. Yakugaku Zasshi 110:950-57
  9. Li HB, Chen F (2005) Preparative isolation and purification of phillyrin from the medicinal plant / Forsythia suspensa by high-speed counter-current chromatography. J Chromatogr A 1083:102-05. doi:10.1016/j.chroma.2005.06.025 CrossRef
  10. Liu YJ, Yang ZQ, Xiao H et al (2004) Effective component in vitro anti-fever blisters virus’s experimental study on the Chinese medicine fructus forsythia. J hubei Coll TCM 6:36-7. doi:cnki:sun:hzxx.0.2004-01-014
  11. Rouge N, Buri P, Doelker E (1996) Drug absorption sites in the gastrointestinal tract and dosage forms for site-specific delivery. Int J Pharma 136:117-39. doi:10.1016/0378-5173(96)85200-8 CrossRef
  12. Sato Y, Kumazawa N, Suzuki M et al (1991) Studies on chemical protectors against radiation. XXXIII. Protective mechanisms of various compounds against skin injury induced by radiation. Yakugaku Zasshi 111:51-8
  13. Schinella GR, Tournier HA, Prieto JM et al (2002) Synthesis and opioid activity of new fentanyl analogs. Life Sci 9:1023-033 CrossRef
  14. Vanthanouvong V, Hogman M, Roomans GM (2003) In vitro and in situ experimental model for X-ray microanalysis of intestinal epithelium. Microsc Res Techniq 62:211-17. doi:10.1002/jemt.10359 CrossRef
  15. Yang TM, Zhang ZH, Zhang H et al (2003) Study on the anti-bacteria function of water extraction fructus forsythia. J Lanzhou Med Coll 9:40-2. doi:cnki:sun:lzyx.0.2003-01-023
  16. Yoshida T, Sakane N, Wakabayashi Y et al (1995) Thermogenic anti-obesity effects of bofu-tsusho-san in MSG-obese mice. Int J Obes Relat Metab Disord 19:717-22
  17. Zhao WH, Shi RB, Liu B et al (2005) Determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC. Zhongguo Zhong Yao Za Zhi 30:36-9. doi:cnki:sun:zgzy.0.2005-01-010
  
• 作者单位：Yun-xia Li (1)
  Cheng Peng (1)
  Liang-hong Ye (1)
  Ruo-qi Zhang (2)
  Xue-hua Jiang (2)
  
  1. Pharmacy College, Chengdu University of Traditional Chinese Medicine; The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine; State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu, 610075, People’s Republic of China
  2. Department of Clinical Pharmacy, West China School of Pharmacy, Sichuan University, No. 17, Section?3, Southern Renmin Road, Chengdu, 610041, People’s Republic of China
  

文摘

In the present study, an in situ rat model was employed to systemically investigate the absorption of phillyrin and forsythiaisde. Three concentrations of phillyrin (0.2, 0.4 and 1.5?mg) were tested and the results showed that phillyrin cannot be absorbed in the digestive tract. The absorption rates of forsythiaside in stomach were 7.773, 7.228 and 6.751%?h? for 0.5, 1 and 2.5?mg, and no significant difference was found in different concentrations. The absorptions of forsythiaside in intestine were investigated in different concentrations and different absorption sites. The mean P% were 6.618, 7.199, 9.210 and 9.747%?h? of forsythiaside in intestine for 0.25, 0.5, 1, 2.5?mg dosage, and the statistical analysis showed that the absorption had no relation with concentration. In addition, in different digestive segments, the mean P% were 7.528, 8.382, 8.191, 9.109 and 6.908%?h? for the gastric, duodenum, jejunum, ileum and colon, respectively. No statistical differences of absorption were found for forsythiaside among different digestive segments indicated no specific absorption site was found.