Study of the biosurfactant-producing profile in a newly isolatedRhodococcus ruber strain

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• 作者：Chenggang Zheng (1)
  Shuguang Li (2)
  Li Yu (3)
  Lixin Huang (3)
  Qinghong Wu (3)
  
• 关键词：Rhodococcus ruber ; biomass ; emulsification index ; cellular hydrophobicity ; biosurfactant ; producing profile
• 刊名：Annals of Microbiology
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：59
• 期：4
• 页码：771-776
• 全文大小：280KB
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• 作者单位：Chenggang Zheng (1)
  Shuguang Li (2)
  Li Yu (3)
  Lixin Huang (3)
  Qinghong Wu (3)
  
  1. Institute of Porous Flow and Fluid Mechanics, Graduate University of Chinese Academy of Sciences, 065007, Langfang, China
  2. Coal-bed Gas Company, China National Petroleum Corporation, 100081, Beijing, China
  3. Research Institute of Petroleum Exploitation and Development (Langfang), China National Petroleum Corporation, 065007, Langfang, China
  
• ISSN：1869-2044

文摘

A biosurfactant-producing strain Z25 was isolated from the oil production water in Daqing Oilfield, China. The strain was identified asRhodococcus ruber by 16S rDNA sequencing.Rhodococcus ruber Z25 showed a preference of alkanes as carbon sources for biosurfactant synthesis. The optimal biosurfactant production was achieved at the NaCI concentration of 2.5% at 34 °C. In batch cultivation, R. ruber Z25 exhibited a cell-growth associated biosurfactant-pro ducing process onn-hexadecane and the maximum yield of biosurfactant production was 13.34 g/L at 44 h. The rate of biosurfactant production per unit biomass (R(biosurf/biomass)) was used to characterize the biosurfactant-producing profile. A two-stage biosurfactant-producing profile of R.ruber Z25 was established by the R(biosurf/biomass) curve, exhibiting the biosurfactant production in correlation with cellular hydrophobicity and biomass accumulation. The crude biosurfactant was extracted by methyltert-butyl ether (MTBE) method and only one glycolipid fraction at Rf value of 0.64 was detected by thin layer chromatography (TLC) analysis.