Identification and characterization of the endophytic bacterium Bacillus atrophaeus XW2, antagonistic towards Colletotrichum gloeosporioides

详细信息    查看全文

• 作者：Huayi Huang ; Ziqiang Wu ; Chengming Tian ; Yingmei Liang…
• 关键词：Bacillus atrophaeus ; Antagonistic activity ; Colletotrichum gloeosporioides ; Sterile bacterial lysates ; Volatiles
• 刊名：Annals of Microbiology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：65
• 期：3
• 页码：1361-1371
• 全文大小：1,283 KB
• 参考文献：Alfonzo A, Piccolo SL, Conigliaro G, Ventorino V, Burruano S, Moschetti G (2012) Antifungal peptides produced by Bacillus amyloliquefaciens AG1 active against grapevine fungal pathogens. Ann Microbiol 62(4):1593-599. doi:10.-007/?s13213-011-0415-2 View Article
  Almenar E, Del Valle V, Catala R, Gavara R (2007) Active package for wild strawberry fruit (Fragaria vesca L.). J Agri Food Chem 55(6):2240-245. doi:10.-021/?jf062809m View Article
  Arrebola E, Sivakumar D, Korsten L (2010) Effect of volatile compounds produced by Bacillus strains on postharvest decay in citrus. Biol Control 53(1):122-28. doi:10.-016/?j.?biocontrol.-009.-1.-10 View Article
  Baysal ?, Lai D, Xu HH, Siragusa M, ?al??kan M, Carimi F, Da Silva JAT, T?r M (2013) A proteomic approach provides new insights into the control of soil-borne plant pathogens by Bacillus species. PLoS One 8(1):e53182. doi:10.-371/?j.?pone.-053182 PubMed Central PubMed View Article
  Beck HC, Hansen AM, Lauritsen FR (2003) Novel pyrazine metabolites found in polymyxin biosynthesis by Paenibacillus polymyxa. FEMS Microbiol Lett 220(1):67-3. doi:10.-016/?S0378-1097(03)00054-5 PubMed View Article
  Chan YK, McCormick WA, Seifert KA (2003) Characterization of an antifungal soil bacterium and its antagonistic activities against Fusarium species. Can J Microbiol 49(4):253-62. doi:10.-139/?w03-033 PubMed View Article
  Chaurasia B, Pandey A, Palni LMS, Trivedi P, Kumar B, Colvin N (2005) Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiol Res 160(1):75-1. doi:10.-016/?j.?micres.-004.-9.-13 PubMed View Article
  Cui TB, Chai HY, Jiang LX (2012) Isolation and partial characterization of an antifungal protein produced by Bacillus licheniformis BS-3. Molecules 17(6):7336-347. doi:10.-390/?molecules1706733- PubMed View Article
  Dong XZ, Cai MY, Lu YY, Xie JY, Liu XL (2001) Identification methods of common bacteria. In: Dong XZ (ed) Cai MY(eds)Handbook of common bacteria systematic identify. Beijing Science press, Beijing, pp 349-98
  Ferreira JHS, Matthee FN, Thomas AC (1991) Biological control of Eutypa lata on grapevine by an antagonistic strain of Bacillus subtilis. Phytopathology 81(3):283-87View Article
  Fu G, Huang S, Ye Y, Wu Y, Cen Z, Lin S (2010) Characterization of a bacterial biocontrol strain B106 and its efficacies on controlling banana leaf spot and post-harvest anthracnose diseases. Biol Control 55(1):1-0. doi:10.-016/?j.?biocontrol.-010.-5.-01 View Article
  Furuya S, Mochizuki M, Aoki Y, Kobayashi H, Takayanagi T, Shimizu M, Suzuki S (2011) Isolation and characterization of Bacillus subtilis KS1 for the biocontrol of grapevine fungal diseases. Biocontrol Sci Tech 21(6):705-20. doi:10.-080/-9583157.-011.-74208 View Article
  Gao SP, Lewis GD, Ashokkumar M, Hemar Y (2014) Inactivation of microorganisms by low-frequency high-power ultrasound: 1 Effect of growth phase and capsule properties of the bacteria. Ultrason Sonochem 21(1):446-53. doi:10.-016/?j.?ultsonch.-013.-6.-06 PubMed View Article
  Guan S, Sattler I, Lin W, Guo D, Grabley S (2005) P-aminoacetophenonic acids produced by a mangrove endophyte: Streptomyces griseus subsp. J Nat Prod 68(8):1198-200. doi:10.-021/?np0500777 PubMed View Article
  He W, Yang W, Shen RX (1991) The preliminary study on anthracnose of Populus?×-em class="EmphasisTypeItalic">beijingensis. For Pest and Dis 4:7-
  He W, Shen RX, Yang W, Wang YY, Tong ZL (1993) Chemical control the anthracnose of Populus?×-em class="EmphasisTypeItalic">beijingensis. For Pest and Dis 2:15-7
  Ju R, Zhao Y, Li J, Jiang H, Liu P, Yang T, Bao Z, Zhou B, Zhou X, Liu X (2014) Identification and evaluation of a potential biocontrol agent, Bacillus subtilis, against Fusarium sp. in apple seedlings. Ann Microbiol 64:377-82. doi:10.-007/?s13213-013-0672-3 View Article
  Kai M, Effmert U, Berg G, Piechulla B (2007) Volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen rhizoctonia solani. Arch Microbiol 187(5):351-60. doi:10.-007/?s00203-006-0199-0 PubMed View Article
  Kai M, Haustein M, Molina F, Petri A, Scholz B, Piechulla B (2009) Bacterial volatiles and their action potential. Appl Microbiol Biotechnol 81(6):1001-012. doi:10.-007/?s00253-008-1760-3 PubMed View Article
  Li Z, Liang YM, Tian CM (2012) Characterization of the causal agent of poplar anthracnose occurring in the Beijing region. Mycotaxon 120(1): 277-86. doi:10.-248/-20.-77
  Li J, Yang Q, Zhao L, Zhang SM, Wang YX, Zhao XY (2009) Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. Univ Sci B 10(4):264-72. doi:10.-631/?jzus.?B0820341 View Article
  Li BQ, Lu XY, Guo QG, Qian CE, Li SZ, Ma P (2010) Isolation and identification of lipopeptides and volatile compounds produced by Bacillus subtilis strain BAB-1. Sci Agr
• 作者单位：Huayi Huang (1)
  Ziqiang Wu (1)
  Chengming Tian (1)
  Yingmei Liang (2)
  Chongjuan You (1)
  Lei Chen (1)
  
  1. The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, No.35, Tsinghua Eastern Road, Haidian District, Beijing, 100083, China
  2. Museum of Beijing Forestry University, Beijing, 100083, China
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

Bacillus atrophaeus XW2 was isolated from healthy poplar leaves and exhibited a strong inhibitory effect against Colletotrichum gloeosporioides, a predominant fungus causing poplar anthracnose. The culture filtrate and bacterial lysate of XW2 were antagonistic against hyphal growth and spore germination. The average diameter of the inhibition zones of hyphal growth were 22.3?mm for the culture filtrate and 12.1?mm for the bacterial lysate. The average inhibition rates of spore germination after 24?h were 94.6?% for the culture filtrate and 88.8?% for the bacterial lysate. In the presence of both culture extracts, hyphae developed vacuoles and swelling, and abnormalities were observed in 100?% of germinated spores. Lipopeptides isolated from culture filtrate and bacterial lysate had high antifungal activities against C. gloeosporioides. The average diameter of the inhibition zones were 23.2?mm for culture filtrate and 11.8?mm for bacterial lysate. Crude proteins isolated from culture filtrate and bacterial lysate also showed good inhibition qualities. The average diameters for the zones of inhibition were 22.3?mm for culture filtrate and 13.4?mm for bacterial lysate. Volatiles produced by XW2 inhibited hyphal growth of C. gloeosporioides by 60.2?% and were antagonistic against the germination of C. gloeosporioides spores after 3?days. Greenhouse studies revealed that XW2 had a 49.1?% efficacy in controlling poplar anthracnose 12?days after exposure to the pathogen. We consider that Bacillus atrophaeus XW2 is a promising natural biocontrol agent for use against C. gloeosporioides.