An electrochemical immunosensor for the tumor marker α-fetoprotein using a glassy carbon electrode modified with a poly(5-formylindole), single-wall carbon nanotubes, and coated with gold nanoparticles and antibody

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• 作者：Lin Zhang ; Chenxi Li ; Dan Zhao ; Tingting Wu ; Guangming Nie
• 关键词：5 ; Formylindole ; Electrochemical polymerization ; SWNTs ; Nanocomposite ; Biosensor
• 刊名：Microchimica Acta
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：181
• 期：13-14
• 页码：1601-1608
• 全文大小：929 KB
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• 作者单位：Lin Zhang (1)
  Chenxi Li (1)
  Dan Zhao (1)
  Tingting Wu (1)
  Guangming Nie (1)
  
  1. State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China
  
• ISSN：1436-5073

文摘

We report on a label-free electrochemical immunosensor for α-fetoprotein (α-FP). It is based on the use of a glassy carbon electrode that was first modified with conducting poly(5-formylindole) and single-walled carbon nanotubes (P5FIn/SWNTs), and then coated with gold nanoparticles and the respective antibody. The presence of aldehyde groups warrants direct immobilization of the antibody and results in a convenient method for fabricating of the immunosensor. Gold nanoparticles (GNPs) were deposited on the P5FIn/SWNTs composite material, and the modified electrode was applied to the detection of α-FP. The analytical signal is obtained by measuring the change of amperometric response at a typical working voltage of 100?mV before and after the immunoreaction. The detection limit is 200?fg?mL?. The immunosensor is simple, sensitive, specific and reproducible. It has the potential for reliable point-of-care diagnosis of tumor or other diseases. Figure A simple electrochemical immunosensor based on conducting poly(5-formylindole) and single-walled carbon nanotubes composite was fabricated to detect alpha-fetoprotein. The detection limit is 200?fg?mL?. This immunosensor is simple, sensitive, specific and reproducible.