A solid-phase screening method for identification of glycan-binding cells

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• 作者：Yoon-Woo Kim ; Kyoung-Jin Kim ; Cheol-Hwan Hwang…
• 关键词：glycan ; binding cells ; N ; glycan ; pig kidney ; α ; galactose ; immobilization
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：20
• 期：2
• 页码：366-372
• 全文大小：662 KB
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• 作者单位：Yoon-Woo Kim (1)
  Kyoung-Jin Kim (1)
  Cheol-Hwan Hwang (1)
  Yun-Gon Kim (1)
  
  1. Department of Chemical Engineering, Soongsil University, Seoul, 156-743, Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

Glycan immobilization and epitope recognition on a solid support is useful for functional glycoproteomics and analysis of protein-protein interactions. However, the lack of high-throughput experimental methods for the examination of cell-glycan interactions means that identifying glycan-binding cells remains a challenge. Herein, we describe a tool which enables the screening of glycanbinding cells that specifically recognize the glycan epitopes released from the membrane glycoproteins of cells and tissues. Biotinylated pig kidney N-glycans were immobilized on streptavidin-conjugated solid supports and then incubated with human immune cell lines. U937 cells discriminated between the α-galactose (α-Gal) and non-Gal pig N-glycans on the solid support. This screening strategy could aid exploration of cell-protein or cell-cell interactions.