Biostimulation of anaerobic BTEX biodegradation under fermentative methanogenic conditions at source-zone groundwater contaminated with a biodiesel blend (B20)
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- 作者：Débora Toledo Ramos (1) <br> Márcio Luis Busi da Silva (2) <br> Helen Simone Chiaranda (1) <br> Pedro J. J. Alvarez (3) <br> Henry Xavier Corseuil (1) <br>
- 关键词：Acetate ; Biodiesel ; Biostimulation ; BTEX ; Methanogenic ; Natural attenuation
- 刊名：Biodegradation
- 出版年：2013
- 出版时间：June 2013
- 年：2013
- 卷：24
- 期：3
- 页码：333-341
- 全文大小：536KB
- 参考文献：1. Ahring BK, Westermann P (1988) Product inhibition of butyrate metabolism by acetate and hydrogen in a thermophilic coculture. Appl Environ Microbiol 54(10):2393-397 <br> 2. Alvarez PJJ, Illman WA (2006) Bioremediation and natural attenuation: process fundamentals and mathematical models. Wiley, Hoboken <br> 3. American Public Health Association (1992) Standard methods for the examination of water and wastewater. American Public Health Association, American Water Works Association and Water Pollution Control Federation, Washington, DC <br> 4. Anderson RT, Rooney-Varga JN, Gaw CV, Lovley DR (1998) Anaerobic benzene oxidation in the Fe(III) reduction zone of petroleum-contaminated aquifers. Environ Sci Technol 32(9):1222-229 CrossRef <br> 5. Anderson RT, Vrionis HA, Ortiz-Bernad I, Resch CT, Long PE, Dayvault R, Karp K, Marutzky S, Metzler DR, Peacock A, White DC, Lowe M, Lovley DR (2003) Stimulating the in situ activity of / Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer. Appl Environ Microbiol 69(10):5884-891 CrossRef <br> 6. Bradley JE, McInerney MJ (2002) Anaerobic microbial metabolism can proceed close to thermodynamic limits. Nature 415:454-56 CrossRef <br> 7. Brazil Law 11.097 from January 13, (2005) Disp?e sobre a introdu??o do biodiesel na matriz energética brasileira, altera as Leis nos 9.478, de 6 de agosto de 1997, 9.847, de 26 de outubro de 1999 e 10.636 de 30 de dezembro de 2002; e dá outras providências. (Refers to the introduction of biodiesel in Brazilian energy matrix, alters the Laws 9.478 from August, 6, 1997; 9.847 from October, 26, 1999 and 10.636 from December, 30, 2002; and makes other provisions) <br> 8. Brazil Ministry of Mines and Energy. National Council of Energy Policy (CNPE), (2009) Estabelece em cinco por cento, em volume, o percentual mínimo obrigatório de adi??o de biodiesel ao óleo diesel comercializado ao consumidor final, de acordo com o disposto no art.o 2o da Lei no 11.097, de 13 de janeiro de 2005. (Establishes up to five percent by volume the minimum percentage required for the addition of biodiesel to diesel oil sold to final consumers, in accordance with the provisions of art. 2 of Law 11.097 from January 13, 2005). Resolution No. 6, September 16, 2009 <br> 9. Bryant M, Campbell L, Reddy C, Crabill M (1977) Growth of / Desulfovibrio in lactate or ethanol media low in sulfate in association with H
- 作者单位：Débora Toledo Ramos (1) <br> Márcio Luis Busi da Silva (2) <br> Helen Simone Chiaranda (1) <br> Pedro J. J. Alvarez (3) <br> Henry Xavier Corseuil (1) <br><br>1. Department of Sanitary and Environmental Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil <br> 2. EMBRAPA, BR153 Km 110, P.O. Box 21, Concórdia, SC, 89700-000, Brazil <br> 3. Department of Civil and Environmental Engineering, Rice University, Houston, TX, USA <br>
- ISSN：1572-9729
文摘
Field experiments were conducted to assess the potential for anaerobic biostimulation to enhance BTEX biodegradation under fermentative methanogenic conditions in groundwater impacted by a biodiesel blend (B20, consisting of 20?% v/v biodiesel and 80?% v/v diesel). B20 (100?L) was released at each of two plots through an area of 1?m2 that was excavated down to the water table, 1.6?m below ground surface. One release was biostimulated with ammonium acetate, which was added weekly through injection wells near the source zone over 15?months. The other release was not biostimulated and served as a baseline control simulating natural attenuation. Ammonium acetate addition stimulated the development of strongly anaerobic conditions, as indicated by near-saturation methane concentrations. BTEX removal began within 8?months in the biostimulated source zone, but not in the natural attenuation control, where BTEX concentrations were still increasing (due to source dissolution) 2?years after the release. Phylogenetic analysis using quantitative PCR indicated an increase in concentration and relative abundance of Archaea (Crenarchaeota and Euryarchaeota), Geobacteraceae (Geobacter and Pelobacter spp.) and sulfate-reducing bacteria (Desulfovibrio, Desulfomicrobium, Desulfuromusa, and Desulfuromonas) in the biostimulated plot relative to the control. Apparently, biostimulation fortuitously enhanced the growth of putative anaerobic BTEX degraders and associated commensal microorganisms that consume acetate and H2b>, and enhance the thermodynamic feasibility of BTEX fermentation. This is the first field study to suggest that anaerobic-methanogenic biostimulation could enhance source zone bioremediation of groundwater aquifers impacted by biodiesel blends.