Differential transcript accumulation in chickpea during early phases of compatible interaction with a necrotrophic fungus Ascochyta rabiei

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• 作者：Purnima Jaiswal (12)
  Jyothi Reddy Cheruku (1)
  Kamal Kumar (1)
  Saurabh Yadav (1)
  Archana Singh (1)
  Pragati Kumari (1)
  Sunil Chandra Dube (3)
  Kailash C. Upadhyaya (2)
  Praveen Kumar Verma (1) praveen_verma@nipgr.res.in
• 关键词：Chickpea &#8211 ; Cicer arietinum &#8211 ; Suppression subtractive hybridization (SSH) &#8211 ; Ascochyta rabiei &#8211 ; Compatible interactions &#8211 ; Induced immunity
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：39
• 期：4
• 页码：4635-4646
• 全文大小：543.5 KB
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• 作者单位：1. Plant Immunity Laboratory, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067 India2. School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067 India3. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

The initial phases of the disease establishment are very crucial for the compatible interactions. Pathogens must overcome the responses generated by the host for the onset of disease invasion. The compatible interaction is inadequately represented in plant-pathogen interaction studies. To gain broader insight into the early responses elicited by chickpea blight fungus Ascochyta rabiei during compatible interaction; we isolated early responsive genes of chickpea using PCR based suppression subtractive hybridization (SSH) strategy. We obtained ~250 unique genes after homology search and redundancy elimination. Based on their potential cellular functions, these genes were broadly classified into eleven different categories viz. stress, signaling, gene regulation, cellular metabolism and genes of unknown functions. Present study revealed few unexpected genes which have a possible role in induced immunity and disease progression. We employed macroarray, northern blot, real-time PCR and cluster analysis to develop transcript profiles. Most of the genes analyzed were early induced and were transcriptionally upregulated upon 24 h post inoculation. Our approach has rendered the isolation of early responsive genes involved in signaling and regulation of metabolic changes upon fungal infection. The information obtained will help to dissect the molecular mechanisms during compatible chickpea–Ascochyta interactions.