Development of 24 new microsatellite markers in the Crested Serpent Eagle (Spilornis cheela hoya)

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• 作者：Hsin-Hsin Hsu (1)
  Shih-Torng Ding (1)
  Yi-Ying Chang (1)
  Ming-Chieh Chao (2)
  Hsien-Shao Tsao (2)
  Fang-Tse Chan (3)
  Chi-Chen Hsu (1)
  Hsiao-Wei Yuan (4)
  Pei-Hwa Wang (1)
  
• 关键词：Crested Serpent Eagle (Spilornis cheela) ; Genetic structure ; Selective hybridization ; Microsatellite markers
• 刊名：Conservation Genetics Resources
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：5
• 期：2
• 页码：417-420
• 全文大小：201KB
• 参考文献：1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-10
  2. Banhos A, Hrbek T, Gravena W, Sanaiotti T, Farias I (2008) Genomic resources for the conservation and management of the Harpy eagle ( / Harpia harpyja, falconiformes, accipitridae). Genet Mol Biol 31:146-54 CrossRef
  3. Chang YY, Chao MC, Ding ST, Lin E-C, Tsao HS, Yuan HW, Wang PH (2012) Development of microsatellite markers in an ungulate mammal, the Formosan serow ( / Capricornis swinhoei). Conserv Genet Resour 4:755-57 CrossRef
  4. Del Hoyo J, Elliott A, Sargatal J, Cabot J (1992) Handbook of the birds of the world, Vol. 2. Lynx Edicions, Barcelona, pp 52-05
  5. Ewing B, Green P (1998) Base-calling of automated sequence traces using phred. II. Error probabilityies. Genome Res 8:186-94
  6. Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequence traces using phred. I. Accuracy assessment. Genome Res 8:175-85
  7. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47-0
  8. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat and automated, locus-specific primer design. Mol Ecol Resour 8:92-4 CrossRef
  9. Ferguson-Lees J, Christie DA (2001) Raptors of the world. Houghton Mifflin Harcourt, Boston, pp 457-60
  10. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Meth Enzymol 395:202-22 CrossRef
  11. Hailer F, Gautschi B, Helander B (2005) Development and multiplex PCR amplification of novel microsatellite markers in the Whitetailed Sea Eagle, / Haliaeetus albicilla (Aves: Falconiformes, Accipitridae). Mol Ecol Notes 5:938-40 CrossRef
  12. IUCN (2009) IUCN red list categories and criteria. IUCN Species Survival Commission. IUCN, Gland
  13. Johnson J, Tingay RE, Culver M, Hailer FM, Clarke L, Mindell DP (2009) Longterm survival despite low genetic diversity in the critically endangered Madagascar fisheagle. Mol Ecol 18:54-3
  14. Martínez-Cruz B, David VA, Godoy JA, Negro JJ, O’Brien SJ, Johnson WE (2002) Eighteen polymorphic microsatellite markers for the highly endangered Spanish imperial eagle ( / Aquila adalberti) and related species. Mol Ecol Notes 2:323-26 CrossRef
  15. Ministry of the Environment (2010) Ministry of the Environment, government of Japan, http://www.env.go.jp/press/press.php?serial=7849
  16. Raymond M, Rousset F (1995) GENEPOP Version 1.2: population genetics software for exact tests and ecumenicism. J Hered 86:248-49
  17. Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz SA, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365-86
  18. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
  19. Takaki Y, Kawahara T, Kitamura H, Endo K, Kudo T (2009) Genetic diversity and genetic structure of Northern goshawk ( / Accipiter gentilis) populations in eastern Japan and central asia. Conserv Genet 10:269-79 CrossRef
  20. Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular biology and biotechnology centre. University of Alberta, Canada
  
• 作者单位：Hsin-Hsin Hsu (1)
  Shih-Torng Ding (1)
  Yi-Ying Chang (1)
  Ming-Chieh Chao (2)
  Hsien-Shao Tsao (2)
  Fang-Tse Chan (3)
  Chi-Chen Hsu (1)
  Hsiao-Wei Yuan (4)
  Pei-Hwa Wang (1)
  
  1. Department of Animal Science and Technology, National Taiwan University, No. 50, Lane155, Sec. 3, Keelung Rd., Taipei, 10673, Taiwan
  2. Taipei Zoo, No. 30, Sec. 2, Xinguang Rd., Taipei, 11656, Taiwan
  3. Endemic Species Research Institute, Council of Agriculture, No. 1, Ming-shen East Rd., Chichi Township, Nantou County, 552, Taiwan
  4. School of Forestry and Resource Conservation, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan
  

文摘

Twenty-four microsatellite markers were developed for the Crested Serpent Eagle (Spilornis cheela hoya) composing a new marker set. The markers were tested on 61 Crested Serpent Eagle individuals. The number alleles ranged from 2 to 8 per locus (average?=?3.8), and the effective number of alleles ranged from 1.13 to 6.07 (average?=?2.34). In the new marker set, there were 11 markers with high polymorphism (PIC?>?0.5), and the average HE and HO over all loci was 0.50 and 0.49, respectively. The results showed that the new marker set was highly polymorphic as an individual genetic marker compared to the existing cross-species markers previously used for population genetic structure monitoring of the Taiwan Crested Serpent Eagle. In conclusion, the microsatellite marker set developed can be applied as a molecular tool to investigate the genetic structure or phylogeny of the Crested Serpent Eagle.