Preparation of a thermo- and pH-sensitive nanofibrous scaffold with embedded chitosan-based nanoparticles and its evaluation as a drug carrier

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• 作者：Chih-Hao Huang (1)
  Ting-Yun Kuo (1)
  Chia-Fen Lee (2)
  Chun-Hsun Chu (3)
  Hsyue-Jen Hsieh (1)
  Wen-Yen Chiu (1) (4) (5)
  
• 关键词：Chitosan ; N ; isopropyl acrylamide ; Nanoparticle ; Drug release ; Electrospinning ; NMA crosslinking
• 刊名：Cellulose
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：21
• 期：4
• 页码：2497-2509
• 全文大小：1,125 KB
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• 作者单位：Chih-Hao Huang (1)
  Ting-Yun Kuo (1)
  Chia-Fen Lee (2)
  Chun-Hsun Chu (3)
  Hsyue-Jen Hsieh (1)
  Wen-Yen Chiu (1) (4) (5)
  
  1. Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan, ROC
  2. Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan City, 71710, Taiwan, ROC
  3. Microsystems Technology Center, Industrial Technology Research Institute, Tainan, Taiwan, ROC
  4. Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 10617, Taiwan, ROC
  5. Department of Materials Science and Engineering, National Taiwan University, Taipei, 10617, Taiwan, ROC
  
• ISSN：1572-882X

文摘

Environmentally sensitive poly(N-isopropylacrylamide) (PNIPAAm) nanofibrous scaffolds loaded with a hydrophilic drug were fabricated via an electrospinning process. First, thermally crosslinkable poly(NIPAAm-co-N-methylolacrylamide) (PNN) was synthesized by redox polymerization below the phase transition temperature of PNIPAAm. The phase transition temperature of the PNN copolymer could be altered from 34 to 40?°C by changing the ratio of N-methylolacrylamide (NMA) to NIPAAm. Subsequently, PNN/chitosan nanofibers were electrospun using ethanol/acetic acid/water as a cosolvent. The PNN/chitosan nanofibers were sensitive to both pH and temperature. The fibrous structure of the soaked PNN/chitosan nanofibers was successfully preserved by the crosslinking of NMA. Furthermore, the chitosan-based nanoparticles (NPs) were introduced into the PNN nanofibers (PNN/NPs) to achieve prolonged drug release. The nanoparticles were observed in the PNN nanofibers by transmission electron microscopy. All of the scaffolds examined had high tensile strengths (1.45?MPa or above) and exhibited no significant cytotoxicity toward human fetal skin fibroblasts. Finally, doxycycline hyclate was used as a model drug. The results illustrated that PNN/NPs nanofibrous scaffolds exhibited continuous drug release behavior for up to 1?week, depending on the pH and temperature.