Colchicine derivative as a potential anti-glioma compound

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• 作者：Kuan-Min Fang ; Jun-Jen Liu ; Chun-Chun Li ; Chih-Chi Cheng…
• 关键词：Glioma ; Colchicine ; Autophagy ; Acidic vesicular organelles ; AD1
• 刊名：Journal of Neuro-Oncology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：124
• 期：3
• 页码：403-412
• 全文大小：3,978 KB
• 参考文献：1.Reardon DA, Wen PY (2006) Therapeutic advances in the treatment of glioblastoma: rationale and potential role of targeted agents. Oncologist 11:152-64CrossRef PubMed
  2.Stupp R, Mason WP, van den Bent MJ, Weller M, Fisher B, Taphoorn MJ, Belanger K, Brandes AA, Marosi C, Bogdahn U, Curschmann J, Janzer RC, Ludwin SK, Gorlia T, Allgeier A, Lacombe D, Cairncross JG, Eisenhauer E, Mirimanoff RO, European Organisation for R, Treatment of Cancer Brain T, Radiotherapy G, National Cancer Institute of Canada Clinical Trials G (2005) Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N Engl J Med 352:987-96
  3.Perry J, Okamoto M, Guiou M, Shirai K, Errett A, Chakravarti A (2012) Novel therapies in glioblastoma. Neurol Res Int 2012:428565CrossRef PubMed PubMed Central
  4.Zhou J, Giannakakou P (2005) Targeting microtubules for cancer chemotherapy. Curr Med Chem Anticancer Agents 5:65-1CrossRef PubMed
  5.Marzo I, Naval J (2013) Antimitotic drugs in cancer chemotherapy: promises and pitfalls. Biochem Pharmacol 86:703-10CrossRef PubMed
  6.Jordan MA, Wilson L (2004) Microtubules as a target for anticancer drugs. Nat Rev Cancer 4:253-65CrossRef PubMed
  7.Zhang Q, Liu X, Li X, Li C, Zhou H, Yan B (2013) Antitumor activity of (2E,5Z)-5-(2-hydroxybenzylidene)-2-((4-phenoxyphenyl)imino) thiazolidin-4-one, a novel microtubule-depolymerizing agent, in U87MG human glioblastoma cells and corresponding mouse xenograft model. J Pharmacol Sci 122:223-31CrossRef PubMed
  8.Ahern MJ, Reid C, Gordon TP, McCredie M, Brooks PM, Jones M (1987) Does colchicine work? The results of the first controlled study in acute gout. Aust N Z J Med 17:301-04CrossRef PubMed
  9.Adler Y, Finkelstein Y, Guindo J, Rodriguez de la Serna A, Shoenfeld Y, Bayes-Genis A, Sagie A, Bayes de Luna A, Spodick DH (1998) Colchicine treatment for recurrent pericarditis. A decade of experience. Circulation 97:2183-185CrossRef PubMed
  10.Bhattacharyya B, Panda D, Gupta S, Banerjee M (2008) Anti-mitotic activity of colchicine and the structural basis for its interaction with tubulin. Med Res Rev 28:155-83CrossRef PubMed
  11.Craig DH, Owen CR, Conway WC, Walsh MF, Downey C, Basson MD (2008) Colchicine inhibits pressure-induced tumor cell implantation within surgical wounds and enhances tumor-free survival in mice. J Clin Investig 118:3170-180CrossRef PubMed PubMed Central
  12.De Vincenzo R, Scambia G, Ferlini C, Distefano M, Filippini P, Riva A, Bombardelli E, Pocar D, Gelmi ML, Benedetti Panici P, Mancuso S (1998) Antiproliferative activity of colchicine analogues on MDR-positive and MDR-negative human cancer cell lines. Anticancer Drug Des 13:19-3PubMed
  13.Liu W, Sun P, Yang L, Wang J, Li L, Wang J (2010) Assay of glioma cell responses to an anticancer drug in a cell-based microfluidic device. Microfluid Nanofluid 9:717-25CrossRef
  14.Yang CS, Tzou BC, Liu YP, Tsai MJ, Shyue SK, Tzeng SF (2008) Inhibition of cadmium-induced oxidative injury in rat primary astrocytes by the addition of antioxidants and the reduction of intracellular calcium. J Cell Biochem 103:825-34CrossRef PubMed
  15.Jiang H, White EJ, Conrad C, Gomez-Manzano C, Fueyo J (2009) Autophagy pathways in glioblastoma. Methods Enzymol 453:273-86CrossRef PubMed
  16.Kanzawa T, Kondo Y, Ito H, Kondo S, Germano I (2003) Induction of autophagic cell death in malignant glioma cells by arsenic trioxide. Cancer Res 63:2103-108PubMed
  17.Fang KM, Lin TC, Chan TC, Ma SZ, Tzou BC, Chang WR, Liu JJ, Chiou SH, Yang CS, Tzeng SF (2013) Enhanced cell growth and tumorigenicity of rat glioma cells by stable expression of human CD133 through multiple molecular actions. Glia 61:1402-417CrossRef PubMed
  18.Fang KM, Wang YL, Huang MC, Sun SH, Cheng H, Tzeng SF (2011) Expression of macrophage inflammatory protein-1alpha and monocyte chemoattractant protein-1 in glioma-infiltrating microglia: involvement of ATP and P2X(7) receptor. J Neurosci Res 89:199-11CrossRef PubMed
  19.Endo K, Mizuguchi M, Harata A, Itoh G, Tanaka K (2010) Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae. FEBS Lett 584:2387-392CrossRef PubMed
  20.Biggers JW, Nguyen T, Di X, Gupton JT, Henderson SC, Emery SM, Alotaibi M, White KL Jr, Brown R, Almenara J, Gewirtz DA (2013) Autophagy, cell death and sustained senescence arrest in B16/F10 melanoma cells and HCT-116 colon carcinoma cells in response to the novel microtubule poison, JG-03-14. Cancer Chemother Pharmacol 71:441-55CrossRef PubMed
  21.Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y, Yoshimori T (2000) LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J 19:5720-728CrossRef PubMed PubMed Central
  22.Paglin S, Hollister T, Delohery T, Hackett N, McMahill M, Sphicas E, Domingo D, Yahalom J (2001) A novel response of cancer cells to radiation involves autophagy and for
• 作者单位：Kuan-Min Fang (1)
  Jun-Jen Liu (2)
  Chun-Chun Li (1) (3)
  Chih-Chi Cheng (1)
  Yun-Ti Hsieh (1)
  Kit Man Chai (1)
  Yu-An Lien (1)
  Shun-Fen Tzeng (1) (3) (4)
  
  1. Department of Life Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan, ROC
  2. School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan, ROC
  3. Center for Cell Dynamics, Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan, ROC
  4. Department of Life Sciences, National Cheng Kung University, #1 University Road, Tainan City, 70101, Taiwan, ROC
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  
• 出版者：Springer Netherlands
• ISSN：1573-7373

文摘

Colchicine, an anti-microtubule and antimitotic drug, is a common therapeutically agent for gout, which is thought to have potential anti-tumor effects. Owing to concerns of colchicines poisoning, the development of derivatives with low dose efficacy and less side effects is of obvious interest. In this study, we characterized the inhibitory effects of a colchicine derivative named AD1 on the cell proliferation of human malignant glioblastoma (MG) cell lines, U87MG and U373MG. We found that 50 % of U87MG and U373MG cells were reduced in the cultures after exposure to AD1 for 24 h at 10 and 50 nM, respectively. Moreover, α-tubulin immunostaining indicated that AD1 induced the disruption of the microtubule polymerization in glioma cells with apoptotic features including membrane budding/blebbing or fragmented nuclei. Increased levels of reactive oxygen species (ROS) were also detected in AD1-treated U87MG and U373MG cells compared to that observed in the control culture. Moreover, examination of microtubule-associated protein 1A/1B-light chain 3 (LC3I)/LC3II conversion and acridine orange staining for autophagic vesicles, combined with flow cytometry, showed that treatment with AD1 induced the autophagic pathway in U87MG and U373MG cells. Furthermore, we found that the intermittent intravenous administration of AD1 suppressed glioma growth in rat brain receiving intracerebral injection with rat C6 glioma cells. Taken together, our findings reveal that treatment with AD1 at nanomolar scales can reduce glioma cell viability effectively, with the occurrence of a rise in ROS and cellular autophagy. In conjunction with the observations from in vivo study, the colchicine derivative AD1 has chemotherapeutic potential to suppress glioma progression. Keywords Glioma Colchicine Autophagy Acidic vesicular organelles AD1