New insight into the catalytic properties of rice sucrose synthase

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• 作者：Yu-Chiao Huang ; Erh-Chieh Hsiang ; Chien-Chih Yang ; Ai-Yu Wang
• 关键词：Sucrose synthase ; Molecular modeling ; Site ; directed mutagenesis ; Enzyme kinetics ; Substrate binding
• 刊名：Plant Molecular Biology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：90
• 期：1-2
• 页码：127-135
• 全文大小：1,107 KB
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• 作者单位：Yu-Chiao Huang (1)
  Erh-Chieh Hsiang (1)
  Chien-Chih Yang (1)
  Ai-Yu Wang (1)
  
  1. Department of Biochemical Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

Sucrose synthase (SuS), which catalyzes the reversible conversion of sucrose and uridine diphosphate (UDP) into fructose and UDP-glucose, is a key enzyme in sucrose metabolism in higher plants. SuS belongs to family 4 of the glycosyltransferases (GT4) and contains an E-X₇-E motif that is conserved in members of GT4 and two other GT families. To gain insight into the roles of this motif in rice sucrose synthase 3 (RSuS3), the two conserved glutamate residues (E678 and E686) in this motif and a phenylalanine residue (F680) that resides between the two glutamate residues were changed by site-directed mutagenesis. All mutant proteins maintained their tetrameric conformation. The mutants E686D and F680Y retained partial enzymatic activity and the mutants E678D, E678Q, F680S, and E686Q were inactive. Substrate binding assays indicated that UDP and fructose, respectively, were the leading substrates in the sucrose degradation and synthesis reactions of RSuS3. Mutations on E678, F680, and E686 affected the binding of fructose, but not of UDP. The results indicated that E678, F680, and E686 in the E-X₇-E motif of RSuS3 are essential for the activity of the enzyme and the sequential binding of substrates. The sequential binding of the substrates implied that the reaction catalyzed by RSuS can be controlled by the availability of fructose and UDP, depending on the metabolic status of a tissue.