Electrochemical determination of glutamic pyruvic transaminase using a microfluidic chip
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- 作者：Jing Wang ; Zheng Xu ; Hong-qun Zou ; Shuai-long Hu…
- 关键词：Electrochemical detection ; Microfluidic chip ; Glutamic pyruvic transaminase ; NADH
- 刊名：Microfluidics and Nanofluidics
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：21
- 期：2
- 全文大小：
- 刊物类别：Engineering
- 刊物主题：Engineering Fluid Dynamics; Biomedical Engineering; Analytical Chemistry; Nanotechnology and Microengineering;
- 出版者：Springer Berlin Heidelberg
- ISSN：1613-4990
- 卷排序：21
文摘
The activity of glutamic pyruvic transaminase (GPT) is an important clinical evidence for some acute diseases such as acute hepatopathy and myocardial infarction. Thus, there is a demand for rapid determination of GPT in small formats at point-of-need. Herein, we describe a novel method of electrochemical determination of GPT with microfluidic technique. GPT activity was indirectly determined via the electrochemical (EC) detection of nicotinamide adenine dinucleotide (NADH) produced from the GPT transdeamination reaction. A type of microfluidic chip was developed, in which a passive mixer comprising 100 sub-ribs and a three-electrode strip for EC were integrated. To verify the response to NADH, a series of NADH concentrations varying from 19 µM to 5 mM were calibrated with cyclic voltammetry within the microfluidic chip. And a linear relationship with R2 0.9982 between the peak current and the concentration of NADH was obtained. Then, the GPT activity was determined using the chips containing and not containing a ribs-type mixer. And a linear relationship which contained two sections between the GPT activity and the peak current was obtained. The chip with a ribs-type mixer exhibited the sensitivity of 0.0341 μA U−1 L in the range of 10–50 U L−1 and 0.0236 μA U−1 L in the range of 50–250 U L−1. And the detection limit of the chip with a ribs-type mixer was 9.25 U L−1. The complete detection process of GPT activity within the microfluidic chip was realized, and the time-consuming problem was remarkably improved too.