Ionizing radiations increase the activity of the cell surface glycohydrolases and the plasma membrane ceramide content

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• 作者：Massimo Aureli (1)
  Rosaria Bassi (1)
  Alessandro Prinetti (1)
  Elena Chiricozzi (1)
  Brigida Pappalardi (2)
  Vanna Chigorno (1)
  Nadia Di Muzio (2)
  Nicoletta Loberto (1)
  Sandro Sonnino (1) (3)
  
• 关键词：Ceramide ; Glycosidases ; Plasma membrane ; Apoptosis ; Radiations ; Radiotherapy
• 刊名：Glycoconjugate Journal
• 出版年：2012
• 出版时间：9 - December 2012
• 年：2012
• 卷：29
• 期：8
• 页码：585-597
• 全文大小：581KB
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• 作者单位：Massimo Aureli (1)
  Rosaria Bassi (1)
  Alessandro Prinetti (1)
  Elena Chiricozzi (1)
  Brigida Pappalardi (2)
  Vanna Chigorno (1)
  Nadia Di Muzio (2)
  Nicoletta Loberto (1)
  Sandro Sonnino (1) (3)
  
  1. Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Milan, Italy
  2. Department of Radiotherapy, Hospital San Raffaele, Milan, Italy
  3. Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Università degli Studi di Milano, Via Fratelli Cervi 93, 20090, Segrate, Italy
  
• ISSN：1573-4986

文摘

We detected significant levels of β-glucosidase, β-galactosidase, sialidase Neu3 and sphingomyelinase activities associated with the plasma membrane of fibroblasts from normal and Niemann-Pick subjects and of cells from breast, ovary, colon and neuroblastoma tumors in culture. All of the cells subjected to ionizing radiations showed an increase of the activity of plasma membrane β-glucosidase, β-galactosidase and sialidase Neu3, in addition of the well known increase of activity of plasma membrane sphingomyelinase, under similar conditions. Human breast cancer cell line T47D was studied in detail. In these cells the increase of activity of β-glucosidase and β-galactosidase was parallel to the increase of irradiation dose up to 60?Gy and continued with time, at least up to 72?h from irradiation. β-glucosidase increased up to 17 times and β-galactosidase up to 40 times with respect to control. Sialidase Neu3 and sphingomyelinase increased about 2 times at a dose of 20?Gy but no further significant differences were observed with increase of radiation dose and time. After irradiation, we observed a reduction of cell proliferation, an increase of apoptotic cell death and an increase of plasma membrane ceramide up to 3 times, with respect to control cells. Tritiated GM3 ganglioside has been administered to T47D cells under conditions that prevented the lysosomal catabolism. GM3 became component of the plasma membranes and was transformed into LacCer, GlcCer and ceramide. The quantity of ceramide produced in irradiated cells was about two times that of control cells.