Analysis of the chloroplast proteome in arc mutants and identification of novel protein components associated with FtsZ2

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• 作者：Daniela Gargano (1)
  Jodi Maple-Gr?dem (1) (2)
  Veronika Reisinger (1)
  Lutz Andreas Eichacker (1)
  Simon Geir M?ller (1) (2) (3)
  
• 关键词：2D ; DIGE ; BN ; PAGE ; FtsZ ; Chloroplast division ; LC–MS/MS
• 刊名：Plant Molecular Biology
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：81
• 期：3
• 页码：235-244
• 全文大小：383KB
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• 作者单位：Daniela Gargano (1)
  Jodi Maple-Gr?dem (1) (2)
  Veronika Reisinger (1)
  Lutz Andreas Eichacker (1)
  Simon Geir M?ller (1) (2) (3)
  
  1. Faculty of Science and Technology, Centre for Organelle Research, University of Stavanger, Stavanger, Norway
  2. The Norwegian Centre for Movement Disorders, Stavanger University Hospital, Stavanger, Norway
  3. Department of Biological Sciences, St John’s University, New York, NY, 11439, USA
  
• ISSN：1573-5028

文摘

Chloroplasts are descendants of cyanobacteria and divide by binary fission. The number of chloroplasts is regulated in a cell type-specific manner to ensure that specialized cell types can perform their functions optimally. Several protein components of the chloroplast division apparatus have been identified in the past several years, but how this process is regulated in response to developmental status, environmental signals and stress is still unknown. To begin to address this we undertook a proteomic analysis of three accumulation and replication of chloroplasts mutants that show a spectrum of plastid division perturbations. We show that defects in the chloroplast division process results in changes in the abundance of proteins when compared to wild type, but that the profile of the native stromal and membrane complexes remains unchanged. Furthermore, by combining BN-PAGE with protein interaction assays we show that AtFtsZ2-1 and AtFtsZ2-2 assemble together with rpl12A and EF-Tu into a novel chloroplast membrane complex.