文摘
We have developed a biosensor for highly sensitive and selective determination of the endocrinic disruptor bisphenol A (BPA). It is based on glassy carbon electrode modified with calf thymus DNA and a composited prepared from single walled carbon nanotubes (SWNT) and Nafion. The interaction between BPA and DNA was studied by voltammetry. The binding constant was determined to be 3.55?×-03?M?, and the binding site has a length of 4.3 base pairs. These electrochemical studies provide further information for a better understanding of the toxicity and carcinogenicity of BPA. Under optimal conditions, the biosensor displays a linear electrochemical response to BPA in the 10 nM to 20?μM concentration range, with a detection limit as low as 5.0 nM (at an S/N of 3). The method was successfully applied to the quantification of BPA in leachates from plastic baby bottles. Recoveries range from 94.0?% to 106.0?% which underpins the excellent performance of this SWNT-based DNA sensor. Figure A biosensor based on DNA and single walled carbon nanotubes modified glassy carbon electrode displays a linear electrochemical response to bisphenol A in the 10 nM to 20 μM concentration range, with a detection limit as low as 5.0 nM (at an S/N of 3).