Singlet oxygen affects the activity of the thylakoid ATP synthase and has a strong impact on its γ subunit

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• 作者：Hanno Mahler (1) (2)
  Petra Wuennenberg (3)
  Monica Linder (4)
  Dominika Przybyla (2)
  Christian Zoerb (5)
  Frank Landgraf (2)
  Christoph Forreiter (1)
  
• 关键词：Non ; photochemical quenching ; Oxidative stress ; Protochlorophyllide ; Reactive oxygen species ; Singlet oxygen ; Thylakoid ATP synthase ; Two ; dimensional polyacrylamide gel electrophoresis (2D PAGE)
• 刊名：Planta
• 出版年：2007
• 出版时间：April 2007
• 年：2007
• 卷：225
• 期：5
• 页码：1073-1083
• 全文大小：511KB
• 参考文献：1. Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot (Lond) 91 Spec No:179-94
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• 作者单位：Hanno Mahler (1) (2)
  Petra Wuennenberg (3)
  Monica Linder (4)
  Dominika Przybyla (2)
  Christian Zoerb (5)
  Frank Landgraf (2)
  Christoph Forreiter (1)
  
  1. Department of Plant Physiology, Justus-Liebig Universit?t, Senckenbergstr. 3, Giessen, 35390, Germany
  2. Department of Plant Sciences, ETH Zurich, Universit?tstr. 2, Zurich, 8092, Switzerland
  3. Department of Molecular Plant Physiology, Friedrich-Alexander Universit?t, Staudtstr. 5, Erlangen, 91058, Germany
  4. Department of Biochemistry, Justus-Liebig Universit?t, Friedrichstrasse 24, Giessen, 35392, Germany
  5. Department of Plant Nutrition, Justus-Liebig Universit?t, Heinrich-Buff-Ring 26-32, Giessen, 35392, Germany
  

文摘

Singlet oxygen is reported to have the most potent damaging effect upon the photosynthetic machinery. Usually this reactive oxygen molecule acts in concert with other ROS types under stressful conditions. To understand the specific role of singlet oxygen we took advantage of the conditional flu mutant of Arabidopsis thaliana. In flu, the negative feedback loop is abolished, which blocks chlorophyll biosynthesis in the dark. Therefore high amounts of free protochlorophyllide accumulate during darkness. If flu gets subsequently illuminated, free protochlorophyllide acts as a photosensitiser leading almost exclusively to high amounts of 1O2. Analysing the thylakoid protein pattern by using 2D PAGE and subsequent MALDI-TOF analysis, we could show, in addition to previous described effects on photosystem II, that singlet oxygen has a massive impact on the thylakoid ATP synthase, especially on its γ subunit. Additionally, it could be shown that the activity of the ATP synthase is reduced upon singlet oxygen exposure and that the rate of non-photochemical quenching is affected in flu mutants exposed to 1O2.