Intraoperative use of enriched collagen and elastin matrices with freshly isolated adipose-derived stem/stromal cells: a potential clinical approach for soft tissue reconstruction

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• 作者：Ziyad Alharbi (1) (2)
  Sultan Almakadi (1)
  Christian Opl?nder (1)
  Michael Vogt (3)
  Hans-Oliver Rennekampff (1)
  Norbert Pallua (1)
  
• 关键词：Adipose tissue ; derived stem/stromal cells ; Stromal vascular fraction ; Liposuction ; Fat grafting ; Biomaterials ; Collagen ; based scaffolds ; Regeneration and tissue engineering
• 刊名：BMC Surgery
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：423 KB
• 参考文献：1. Fraser JK, Zhu M, Wulur I, Alfonso Z: Adipose-derived stem cells. / Methods Mol Biol 2008, 449:59-7.
  2. Tholpady SS, Llull R, Ogle RC, Rubin JP, Futrell JW, Katz AJ: Adipose tissue: stem cells and beyond. / Clin Plast Surg 2006,33(1):55-2. CrossRef
  3. Mizuno H: Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review. / J Nihon Med Sch 2009,76(2):56-6. CrossRef
  4. Gimble JM: Adipose tissue-derived therapeutics. / Expert Opin Biol Ther 2003, 3:705-13. CrossRef
  5. Alharbi Z, Opl?nder C, Almakadi S, Fritz A, Vogt M, Pallua N: Conventional vs. micro-fat harvesting: how fat harvesting technique affects tissue-engineering approaches using adipose tissue-derived stem/stromal cells. / J Plast Reconstr Aesthet Surg 2013,66(9):1271-278. CrossRef
  6. Keck M, Haluza D, Lumenta DB, Burjak S, Eisenbock B, Kamolz LP, Frey M: Construction of a multi-layer skin substitute: Simultaneous cultivation of keratinocytes and preadipocytes on a dermal template. / Burns 2011,37(4):626-30. CrossRef
  7. Coleman SR: Structural fat grafts: the ideal filler? / Clin Plast Surg 2001, 28:111-19.
  8. Chang Q, Li J, Dong Z, Liu L, Lu F: Quantitative volumetric analysis of progressive hemifacial atrophy corrected using stromal vascular fraction-supplemented autologous fat grafts. / Dermatol Surg 2013,39(10):1465-473.
  9. Jeon H, Kim J, Yeo H, Jeong H, Son D, Han K: Treatment of diabetic foot ulcer using matriderm in comparison with a skin graft. / Arch Plast Surg 2013,40(4):403-08. CrossRef
  10. Walum E, Hansson E, Harvey AL: In vitro testing of neurotoxicity. / ATLA 1990, 18:153-79.
  11. Li K, Wong D, Hiscott P, Stanga P, Groenewald C, McGalliard J: Trypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: Visual results and histopathological findings. / Br J Ophthalmol 2003, 87:216-19. CrossRef
  12. Ahmed SA, Gogal RM Jr, Walsh JE: A new Rapid and Simple Non-Radioactive assay to Monitor and Determine the proliferation of Lymphocytes: An Alternative to H3-thymidine incorporation assay. / J lmmunol Methods 1994, 170:211-24. CrossRef
  13. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH, Boyd MR: Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay. / Cancer Res 1988, 48:589-01.
  14. The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2482/14/10/prepub
  
• 作者单位：Ziyad Alharbi (1) (2)
  Sultan Almakadi (1)
  Christian Opl?nder (1)
  Michael Vogt (3)
  Hans-Oliver Rennekampff (1)
  Norbert Pallua (1)
  
  1. Department of Plastic, Reconstructive and Hand Surgery - Burn Center, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, Aachen, D-52074, Germany
  2. Division of Plastic Surgery, Specialist Surgery Center, King Abdullah Medical City, Mecca, Kingdom of Saudi Arabia
  3. Two-Photon Microscopy Facility, Interdisciplinary Center for Clinical Research (IZKF), Medical Faculty, RWTH Aachen University, Aachen, Germany
  
• ISSN：1471-2482

文摘

Background Adipose tissue contains a large number of multipotent cells, which are essential for stem cell-based therapies. The combination of this therapy with suitable commercial clinically used matrices, such as collagen and elastin matrices (i.e. dermal matrices), is a promising approach for soft tissue reconstruction. We previously demonstrated that the liposuction method affects the adherence behaviour of freshly isolated adipose-derived stem/stromal cells (ASCs) on collagen and elastin matrices. However, it remains unclear whether freshly isolated and uncultured ASCs could be directly transferred to matrices during a single transplantation operation without additional cell culture steps. Methods After each fat harvesting procedure, ASCs were isolated and directly seeded onto collagen and elastin matrices. Different time intervals (i.e. 1, 3 and 24?h) were investigated to determine the time interval needed for cellular attachment to the collagen and elastin matrices. Resazurin-based vitality assays were performed after seeding the cells onto the collagen and elastin matrices. In addition, the adhesion and migration of ASCs on the collagen and elastin matrices were visualised using histology and two-photon microscopy. Results A time-dependent increase in the number of viable ASCs attached to the collagen and elastin matrices was observed. This finding was supported by mitochondrial activity and histology results. Importantly, the ASCs attached and adhered to the collagen and elastin matrices after only 1?h of ex vivo enrichment. This finding was also supported by two-photon microscopy, which revealed the presence and attachment of viable cells on the upper layer of the construct. Conclusion Freshly isolated uncultured ASCs can be safely seeded onto collagen and elastin matrices for ex vivo cellular enrichment of these constructs after liposuction. Although we observed a significant number of seeded cells on the matrices after a 3-h enrichment time, we also observed an adequate number of isolated cells after a 1-h enrichment time. However, this approach must be optimised for clinical use. Thus, in vivo studies and clinical trials are needed to investigate the feasibility of this approach.