Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts

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• 作者：Iskander M. Ibrahim ; Sujith Puthiyaveetil ; Christine Khan…
• 关键词：Two ; component regulatory system ; Sensor histidine kinase ; Response regulator ; Chloroplast sensor kinase ; Plastoquinone ; Redox regulation ; Chloroplast DNA ; Transcription ; Cyanobacteria ; Photosystem stoichiometry
• 刊名：Photosynthesis Research
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：130
• 期：1-3
• 页码：93-101
• 全文大小：1,206 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5079
• 卷排序：130

文摘

Two-component signal transduction systems mediate adaptation to environmental changes in bacteria, plants, fungi, and protists. Each two-component system consists of a sensor histidine kinase and a response regulator. Chloroplast sensor kinase (CSK) is a modified sensor histidine kinase found in chloroplasts—photosynthetic organelles of plants and algae. CSK regulates the transcription of chloroplast genes in response to changes in photosynthetic electron transport. In this study, the full-length and truncated forms of Arabidopsis CSK proteins were overexpressed and purified in order to characterise their kinase and redox sensing activities. Our results show that CSK contains a modified kinase catalytic domain that binds ATP with high affinity and forms a quinone adduct that may confer redox sensing activity.