Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts

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• 作者：Tom Eeckhaut (1)
  Johan Van Huylenbroeck (1)
  
• 关键词：Aeration ; In vitro ; Liquid medium ; Microcalli ; Regeneration
• 刊名：Acta Physiologiae Plantarum
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：33
• 期：4
• 页码：1547-1551
• 全文大小：230KB
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• 作者单位：Tom Eeckhaut (1)
  Johan Van Huylenbroeck (1)
  
  1. Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit-Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium
  

文摘

This study is a comparison of four methods to induce calli formation in a protoplast culture of Chrysanthemum indicum. Culture in liquid medium (17.6?calli/105 protoplasts) was preferable to culture in solid agarose beads, although the efficiency of the latter could be improved by layering them on glass beads (12.5 vs. 0.83?calli/105 protoplasts). Culture of protoplasts on moistened filter paper was unsuccessful. In the liquid media, microcalli and calli were induced efficiently and easily after 6?weeks. These effects may be explained by reduced toxicity due to cell breakdown and improved aeration.