Elimination of Chrysanthemum stunt viroid (CSVd) from meristem tip culture combined with prolonged cold treatment

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• 作者：Wina Dian Savitri (1141)
  Kyung Il Park (2141)
  Su Min Jeon (1141)
  Mi Young Chung (3141)
  Jeung-Sul Han (4141)
  Chang Kil Kim (1141)
  
• 关键词：amantadine ; CSVd ; free ; low temperature ; nested PCR ; ribavirin
• 刊名：Horticulture, Environment, and Biotechnology
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：54
• 期：2
• 页码：177-182
• 全文大小：201KB
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• 作者单位：Wina Dian Savitri (1141)
  Kyung Il Park (2141)
  Su Min Jeon (1141)
  Mi Young Chung (3141)
  Jeung-Sul Han (4141)
  Chang Kil Kim (1141)
  
  1141. Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, Korea
  2141. Department of Horticulture & Life Science, Yeungnam University, Gyeongsan, 712-749, Korea
  3141. Department of Agricultural Education, Sunchon National University, Sunchon, 540-742, Korea
  4141. Department of Ecological Environment, Kyungpook National University, Sangju, 742-711, Korea
  

文摘

Chrysanthemum production in Korea has recently been greatly affected by the spread of Chrysanthemum stunt viroid (CSVd) infection, necessitating the use of CSVd-free stocks to ensure successful chrysanthemum cultivation. We investigated the effects of low temperature (4°C), antiviral chemicals (ribavirin and amantadine) and a combination of these treatments on CSVd elimination by meristem tip cultures using plantlets that originated from CSVd-infected chrysanthemum ‘Ency- Neither antiviral agents led to CSVd elimination, despite the suppression of meristem tip growth in a concentration dependent manner. However, the CSVd elimination rate increased up to 42.8% when meristem tips were excised after storage at 4°C for two months. The most effective results were obtained from a combination of low temperature for three months at 4°C followed by meristem tip culture on media containing 50 and 100 mg·L? ribavirin. These results suggest that antiviral agents can also be useful for CSVd elimination if their treatment is combined with prolonged periods of low temperature. This is the first report of eradication of viroids from spray type chrysanthemum bred in Korea.