Coupling of Spinosad Fermentation and Separation Process via Two-Step Macroporous Resin Adsorption Method

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• 作者：Fanglong Zhao ; Chuanbo Zhang ; Jing Yin…
• 关键词：Spinosad ; Saccharopolyspora spinosa ; Fermentation ; Separation process ; Coupling
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：176
• 期：8
• 页码：2144-2156
• 全文大小：4,503 KB
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• 作者单位：Fanglong Zhao (1) (2) (3)
  Chuanbo Zhang (1) (2) (3)
  Jing Yin (1) (2) (3)
  Yueqi Shen (1) (2) (3)
  Wenyu Lu (1) (2) (3)
  
  1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
  2. Key Laboratory of system bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, People’s Republic of China
  3. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

In this paper, a two-step resin adsorption technology was investigated for spinosad production and separation as follows: the first step resin addition into the fermentor at early cultivation period to decrease the timely product concentration in the broth; the second step of resin addition was used after fermentation to adsorb and extract the spinosad. Based on this, a two-step macroporous resin adsorption-membrane separation process for spinosad fermentation, separation, and purification was established. Spinosad concentration in 5-L fermentor increased by 14.45?% after adding 50?g/L macroporous at the beginning of fermentation. The established two-step macroporous resin adsorption-membrane separation process got the 95.43?% purity and 87?% yield for spinosad, which were both higher than that of the conventional crystallization of spinosad from aqueous phase that were 93.23 and 79.15?% separately. The two-step macroporous resin adsorption method has not only carried out the coupling of spinosad fermentation and separation but also increased spinosad productivity. In addition, the two-step macroporous resin adsorption-membrane separation process performs better in spinosad yield and purity.