Construction of a bacterial artificial chromosome (BAC) library of common wild rice ( Oryza rufipogon Griff.) for map-based cloning of genes selected during the domestication of rice

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• 作者：Xianran Li ; LuBin Tan ; Haiyan Huang ; Zuofeng Zhu ; Chenji Li ; Songnian Hu and Chuanqing Sun
• 关键词：BAC library ; Common wild rice ; Domestication ; Retrotransposon ; Selected genes
• 刊名：Biotechnology Letters
• 出版年：2008
• 出版时间：March, 2008
• 年：2008
• 卷：30
• 期：3
• 页码：555-561
• 全文大小：249.6 KB

文摘

As a prerequisite for the map-based cloning of genes from common wild rice (Oryza rufipogon Griff.), which plays an important role in the domestication of cultivated rice (O. sativa L.), we constructed a median-insert size bacterial artificial chromosome (BAC) library of the common wild rice isolate, YJCWR, collected from Yuanjiang, Yunnan Province, China. The library consists of 52,992 clones, with an average insert size of 50 kb, and all clones were pooled into 46 three-dimensional super-pools to facilitate library screening through the PCR method. Seventeen candidate clones were isolated by five markers and some clones containing putative target regions were sequenced. Furthermore, in analyzing the sequences of YJCWR, a retrotransposon, SZ-55, that might contribute to the evolution of Oryza was found.