Construction of the physical map of the gpa7 locus reveals that a large segment was deleted during rice domestication

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• 作者：Xianran Li ; Feng Tian ; Haiyan Huang ; Lubin Tan ; Zuofeng Zhu ; Songnian Hu and Chuanqing Sun
• 关键词：Physical map ; Common wild rice ; gpa7 locus ; Domestication
• 刊名：Plant Cell Reports
• 出版年：2008
• 出版时间：June, 2008
• 年：2008
• 卷：27
• 期：6
• 页码：1087-1092
• 全文大小：268.4 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

To facilitate cloning gene(s) underlying gpa7, a deep-coverage BAC library was constructed for an isolate of common wild rice (Oryza rufipogon Griff.) collected from Dongxiang, Jiangxi Province, China (DXCWR). gpa7, a quantitative trait locus corresponding to grain number per panicle, is positioned in the short arm of chromosome 7. The BAC library containing 96,768 clones represents approximate 18 haploid genome equivalents. The contig spanning DXCWR gpa7 was constructed with a series of ordered markers. The putative physical map near the gpa7 locus of another accession of O. rufipogon (Accession: IRGC 105491) was also isolated in silico. Analysis of the physical maps of gpa7 indicated that a segment of about 150 kb was deleted during domestication of common wild rice.