Antimicrobial peptides in the centipede Scolopendra subspinipes mutilans
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- 作者：Won Gi Yoo (1)
Joon Ha Lee (2)
Younhee Shin (1)
Jae-Young Shim (1)
Myunghee Jung (1)
Byeong-Chul Kang (1)
Jaedon Oh (5)
Jiyeon Seong (5)
Hak Kyo Lee (5)
Hong Sik Kong (5)
Ki-Duk Song (5)
Eun-Young Yun (2)
In-Woo Kim (2)
Young-Nam Kwon (2)
Dong Gun Lee (3)
Ui-Wook Hwang (4)
Junhyung Park (1)
Jae Sam Hwang (2) - 关键词：RNA ; seq ; Physicochemical properties ; Antimicrobial peptide ; Scolopendra subspinipes mutilans
- 刊名：Functional & Integrative Genomics
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：14
- 期：2
- 页码：275-283
- 全文大小：
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Joon Ha Lee (2)
Younhee Shin (1)
Jae-Young Shim (1)
Myunghee Jung (1)
Byeong-Chul Kang (1)
Jaedon Oh (5)
Jiyeon Seong (5)
Hak Kyo Lee (5)
Hong Sik Kong (5)
Ki-Duk Song (5)
Eun-Young Yun (2)
In-Woo Kim (2)
Young-Nam Kwon (2)
Dong Gun Lee (3)
Ui-Wook Hwang (4)
Junhyung Park (1)
Jae Sam Hwang (2)
1. Codes Division, Insilicogen, Inc., Suwon, Gyeonggi-do, 441-813, South Korea
2. Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi-do, 441-853, South Korea
5. Genomic Informatics Center, Hankyong National University, Gyeonggi-do, 456-749, South Korea
3. School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Buk-gu, Daegu, 702-701, South Korea
4. Department of Biology, Teachers College, Institute for Phylogenomics and Evolution, Kyungpook National University, Daegu, 702-701, South Korea - ISSN：1438-7948
文摘
The centipede Scolopendra subspinipes mutilans is an environmentally beneficial and medically important arthropod species. Although this species is increasingly applied as a reliable source of new antimicrobial peptides, the transcriptome of this species is a prerequisite for more rational selection of antimicrobial peptides. In this report, we isolated total RNA from the whole body of adult centipedes, S. subspinipes mutilans, that were nonimmunized and immunized against Escherichia coli, and we generated a total of 77,063 pooled contigs and singletons using high-throughput sequencing. To screen putative antimicrobial peptides, in silico analyses of the S. subspinipes mutilans transcriptome were performed based on the physicochemical evidence of length, charge, isoelectric point, and in vitro and in vivo aggregation scores together with the existence of continuous antimicrobial peptide stretches. Moreover, we excluded some transcripts that showed similarity with both previously known antimicrobial peptides and the human proteome, had a proteolytic cleavage site, and had downregulated expression compared with the nonimmunized sample. As a result, we selected 17 transcripts and tested their antimicrobial activity with a radial diffusion assay. Among them, ten synthetic peptides experimentally showed antimicrobial activity against microbes and no toxicity to mouse erythrocytes. Our results provide not only a useful set of antimicrobial peptide candidates and an efficient strategy for novel antimicrobial peptide development but also the transcriptome data of a big centipede as a valuable resource.