Novel six-week protocol for generating functional human connective tissue-type (MCTC) mast cells from buffy coats
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- 作者：Issan Yee San Tam ; Chun Wai Ng ; See-Ying Tam…
- 关键词：Allergy ; Buffy coat ; Culture protocol ; Human mast cell ; Inflammation
- 刊名：Inflammation Research
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：66
- 期：1
- 页码：25-37
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Immunology; Pharmacology/Toxicology; Rheumatology; Allergology; Dermatology; Neurology;
- 出版者：Springer International Publishing
- ISSN：1420-908X
- 卷排序：66
文摘
ObjectiveThe aim of this study was to develop a novel protocol for generating large populations of fully mature and functional human mast cells (HMC) from CD34+ hematopoietic stem cells which require less culturing time than previously reported methods.