One-step RT-LAMP analysis for efficient detection of Odontoglossum ringspot virus from Oncidiinae hybrids (Orchidaceae)

详细信息    查看全文

• 作者：Chi-Chu Tsai ; Chun-Yen Huang ; I-Szu Weng ; Wen-Lin Liu…
• 关键词：Virus detection ; RNA ; dependent RNA polymerase ; RT ; LAMP ; Orchid
• 刊名：Journal of General Plant Pathology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：82
• 期：2
• 页码：89-95
• 全文大小：1,857 KB
• 参考文献：Almasi MA, Jafary H, Moradi A, Zand N, Ojaghkandi MA, Aghaei S (2013) Detection of coat protein gene of the Potato leafroll virus by reverse transcription loop-mediated isothermal amplification. J Plant Pathol Microb 4:156
  Buates S, Bantuchai S, Sattabongkot J, Han ET, Tsuboi T, Udomsangpetch R, Sirichaisinthop J, Tan-Ariya P (2010) Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for clinical detection of Plasmodium falciparum gametocytes. Parasitol Int 59:414–420CrossRef PubMed
  Chan KW, Liu PC, Yang WC, Kuo J, Chang CLT, Wang CY (2012) A novel loop-mediated isothermal amplification approach for sex identification of Columbidae birds. Theriogenology 78:1329–1338CrossRef PubMed
  Deyong Z, Willingmann P, Heinze C, Adam G, Pfunder M, Frey B, Frey JE (2005) Differentiation of Cucumber mosaic virus isolates by hybridization to oligonucleotides in a microarray format. J Virol Methods 123:101–108CrossRef PubMed
  Eun AJC, Wong SM (2000) Molecular beacons: a new approach to plant virus detection. Phytopathology 90:269–275CrossRef PubMed
  Eun AJC, Seoh ML, Wong SM (2000) Simultaneous quantitation of two orchid viruses by the TaqMan real-time RT-PCR. J Virol Methods 87:151–160CrossRef PubMed
  Fukuta S, Iida T, Mizukami Y, Ishida A, Ueda J, Kanbe M, Ishimoto Y (2003) Detection of Japanese yam mosaic virus by RT-LAMP. Arch Virol 148:1713–1720CrossRef PubMed
  Fukuta S, Ohishi K, Yoshida K, Mizukami Y, Ishida A, Kanbe M (2004) Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum. J Virol Methods 121:49–55CrossRef PubMed
  Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98
  Hsu TH, Gwo JC, Lin KH (2012) Rapid sex identification of papaya (Carica papaya) using multiplex loop-mediated isothermal amplification (mLAMP). Planta 236:1239–1246CrossRef PubMed
  Hu JS, Ferreira S, Wang M, Xu MQ (1993) Detection of Cymbidium mosaic virus, Odontoglossum ringspot virus, Tomato spotted wilt virus, and potyviruses infecting orchids in Hawaii. Plant Dis 77:464–468CrossRef
  Huang CH, Lai GH, Lee MS, Lin WH, Lien YY, Hsueh SC, Kao JY, Chang WT, Lu TC, Lin WN, Chen HJ, Lee MS (2010) Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of chicken anaemia virus. J Appl Microbiol 108:917–924CrossRef PubMed
  Jensen DD, Gold AH (1951) A virus ring spot of Odontoglossum orchid: symptoms, transmission, and electron microscopy. Phytopathology 41:648–653
  Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948CrossRef PubMed
  Lee SC, Chang YC (2006) Multiplex RT-PCR detection of two orchid viruses with an internal control of plant nad5 mRNA. Plant Pathol Bull 15:187–196
  Lee MS, Yang MJ, Hseu YC, Lai GH, Chang WT, Hsu YH, Lin MK (2011) One-step reverse transcription loop-mediated isothermal amplification assay for rapid detection of Cymbidium mosaic virus. J Virol Methods 173:43–48CrossRef PubMed
  Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63PubMedCentral CrossRef PubMed
  Okemura AK, Kamemoto H, Ishii M (1984) Incidence and expression of cymbidium mosaic virus in Dendrobium hybrids. Hawaii Inst Trop Agric Hum Resour Res Ext Ser 33:3–13
  Qin WT, Wang ZG, Zhang H (2013) The progress of loop-mediated isothermal amplification (LAMP) and its application in detection of plant virus (In Chinese with English abstract). Chin Agric Sci Bull 29:170–174
  Ryu KH, Park WM (1995) The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA. Arch Virol 140:1577–1587CrossRef PubMed
  Thornberry HH, Phillippe MR (1964) Orchid disease: cattleya blossom brown necrotic streak. Plant Dis Rep 48:936–940
  Tsutsumi N, Yanagisawa H, Fujiwara Y, Ohara T (2010) Detection of Potato spindle tuber viroid by reverse transcription loop-mediated isothermal amplification. Res Bull Plant Prot Japan 46:61–67
  Varga A, James D (2006) Use of reverse transcription loop-mediated isothermal amplification for the detection of Plum pox virus. J Virol Methods 138:184–190CrossRef PubMed
  Wong SM, Chng CG, Lee YH, Tan K, Zettler FW (1994) Incidence of cymbidium mosaic and odontoglossum ringspot viruses and their significance in orchid cultivation in Singapore. Crop Protect 13:235–239CrossRef
  Yamane K, Oyama K, Iuchi E, Ogawa H, Suzuki T, Natsuaki T (2008) RT-PCR Detection of Odontoglossum ringspot virus, Cymbidium mosaic virus and tospoviruses and association of infections with leaf-yellowing symptoms in Phalaenopsis. J Phytopathol 156:268–273CrossRef
  
• 作者单位：Chi-Chu Tsai (1) (2)
  Chun-Yen Huang (1) (2) (3)
  I-Szu Weng (1) (2) (3)
  Wen-Lin Liu (1)
  Chang-Hung Chou (3)
  
  1. Kaohsiung District Agricultural Research and Extension Station, Pingtung, 908, Taiwan
  2. National Pingtung University of Science and Technology, Pingtung, 912, Taiwan
  3. Research Center for Biodiversity, China Medical University, Taichung, 404, Taiwan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Agriculture
  Microbiology
  
• 出版者：Springer Japan
• ISSN：1610-739X

文摘

Odontoglossum ringspot virus (ORSV) is one of the most serious pathogens in the orchid industry. The production of virus-free orchid seedlings is the most efficient strategy to counter this virus problem. Therefore, an easy, efficient process to detect viruses is important to the orchid industry. One-step reverse transcription (RT) loop-mediated isothermal amplification (LAMP) technique was developed to detect ORSV in Oncidiinae hybrids. RT-LAMP analysis primers were designed based on the conserved regions of the RNA-dependent RNA polymerase (RdRp) gene among ORSV isolates. RT-LAMP reaction conditions were optimized to detect ORSV from Oncidiinae hybrids. This one-step RT-LAMP method proved to be easy, efficient, sensitive, and inexpensive for detecting ORSV in orchids and is the first application of a one-step RT-LAMP to detect ORSV.