MiR-34a suppresses ovarian cancer proliferation and motility by targeting AXL

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• 作者：Rui Li ; Xuejun Shi ; Fengyu Ling ; Chunguang Wang ; Junxia Liu ; Wei Wang…
• 关键词：Ovarian cancer ; MiR ; 34a ; AXL ; P53 ; Proliferation ; Migration
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：36
• 期：9
• 页码：7277-7283
• 全文大小：938 KB
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• 作者单位：Rui Li (1)
  Xuejun Shi (1)
  Fengyu Ling (1)
  Chunguang Wang (1)
  Junxia Liu (1)
  Wei Wang (1)
  Ming Li (1)
  
  1. Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Increasing evidence has suggested that dysregulation of microRNAs (miRNAs) could contribute to tumor progression. The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in various cancers; however, the effect of miR-34a on the ovarian cancer cells remains unclear. The aim of the paper was to study the expression of miR-34a in ovarian cancer and miR-34a’s relation to the cell proliferation and metastasis in ovarian cancer in vitro. miR-34a expression was determined by quantitative RT-PCR in a panel of 60 human ovarian cancer samples. Functional characterization of miR-34a was accomplished by reconstitution of miR-34a expression in ovarian cancer cells by determining changes in proliferation, migration, and invasion. Our results showed that miR-34a is downregulated in ovarian cancer tissues compared with the corresponding adjacent non-neoplastic tissues, and the expression level of miR-34a was significantly lower in ovarian cancer cell lines in comparison with normal human fallopian tube epithelial cell line. The 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay revealed significant cell proliferation inhibition in miR-34a transfectant compared with the control from HO8910 and SKOV3 cells, which displayed lowest expressions of miR-34a. Furthermore, the transwell assay also showed significant cell migration inhibition in miR-34a transfectant, compared with cell lines transfected with NC. Overexpression of miR-34a led to the inhibition of AXL expression, indicating that AXL is a target gene for miR-34a. Our data suggest that miR-34a may function as a tumor suppressor through repression of oncogenic AXL in ovarian cancer.