miR-124 Represses ROCK1 Expression to Promote Neurite Elongation Through Activation of the PI3K/Akt Signal Pathway

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• 作者：Xi Gu (1)
  Siying Meng (1)
  Shuhu Liu (1)
  Chunhong Jia (2)
  Yingying Fang (1)
  Shuji Li (1)
  Congcong Fu (1)
  Qiancheng Song (2)
  Lifang Lin (1)
  Xuemin Wang (1)
  
• 关键词：miR ; 124 ; ROCK1 ; Neurite elongation ; PI3K/Akt
• 刊名：Journal of Molecular Neuroscience
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：52
• 期：1
• 页码：156-165
• 全文大小：1,333 KB
• 作者单位：Xi Gu (1)
  Siying Meng (1)
  Shuhu Liu (1)
  Chunhong Jia (2)
  Yingying Fang (1)
  Shuji Li (1)
  Congcong Fu (1)
  Qiancheng Song (2)
  Lifang Lin (1)
  Xuemin Wang (1)
  
  1. Department of Neurobiology, Southern Medical University, Guangzhou, Guangdong Province, China
  2. Department of Cell Biology, Southern Medical University, Guangzhou, Guangdong Province, China
  
• ISSN：1559-1166

文摘

Recent studies have demonstrated an important role for miR-124, the most abundant and well-conserved brain-specific microRNA(miRNA), in promoting neurite outgrowth and elongation during neuronal differentiation. This miRNA’s target genes and the mechanisms that execute this role remain unclear. In this study, we identified ROCK1, a small GTPase Rho kinase, as a direct target of miR-124 for regulating neurite elongation. miR-124 significantly inhibited ROCK1 expression in M17 cells. Inhibiting ROCK1 promoted neurite elongation, and the overexpression of ROCK1 strongly repressed the neurite elongation-enhancing effect of miR-124 in M17 cells. We determined that Akt functions as a novel ROCK1 downstream effector in regulating neurite outgrowth and elongation.