Responses of Fungi Community Structure to the Presence of Pesticide Chlorpyrifos in the Soil Planting Brassica Juncea (L.) Czerniak

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• 作者：Chunping Huang ; Xin Chen ; Jiang Li ; Fuzhong Wu ; Wanqin Yang…
• 关键词：Chlorpyrifos ; Fungal community ; Agricultural practices ; qPCR ; DGGE
• 刊名：Water, Air, and Soil Pollution
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：227
• 期：2
• 全文大小：732 KB
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• 作者单位：Chunping Huang (1) (2)
  Xin Chen (2)
  Jiang Li (2)
  Fuzhong Wu (1)
  Wanqin Yang (1)
  Jian Zhang (1)
  
  1. Key Laboratory of Ecological Forestry Engineering, Institute of Ecological Forestry, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China
  2. College of life Sciences, Sichuan Normal University, Chengdu, 610101, People’s Republic of China
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Atmospheric Protection, Air Quality Control and Air Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Terrestrial Pollution
  Hydrogeology
  
• 出版者：Springer Netherlands
• ISSN：1573-2932

文摘

Because of its persistent usage and broad-spread applicability, chlorpyrifos with high potential damage to non-target organism can be found widely in the environment. However, the relevant researches about the effects of chlorpyrifos on soil fungi, an important part of microorganisms in the planting soil, are very limited, especially when chlorpyrifos is applied in actual agricultural practices. In this study, the soils, planted with Brassica juncea (L.) Czerniak (big mustard), treated with chlorpyrifos were analyzed during vegetable growth to be harvested. The effects of chlorpyrifos on fungal abundance and community structure were determined by quantitative polymerase chain reaction (qPCR) and denaturing gradient gel electrophoresis (DGGE). The results revealed that chlorpyrifos was removed only 15.20 % on the 15th day after being sprayed. Chlorpyrifos caused inhibition on soil fungi diversity and fungal abundance significantly decreased from the early days after application. Furthermore, an obvious change in fungal community structure was found in the treatments compared with the controls, especially a significant change of Fusarium sp., which maintained stable abundance in the controls but fell sharply when chlorpyrifos started to be used and then significantly increased in the treatments, even over the controls finally. In contrast to the controls, the effects from chlorpyrifos could change soil fungal structure by affecting soil pH, while the other soil physicochemical properties without significant influence from chlorpyrifos.