Mossy Fibers Terminate Directly Within Purkinje Cell Zones During Mouse Development

详细信息    查看全文

• 作者：Roy V. Sillitoe
• 关键词：Spinocerebellar ; Zones ; Postnatal ; Circuitry ; Neural tracing ; Transynaptic
• 刊名：The Cerebellum
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：15
• 期：1
• 页码：14-17
• 全文大小：913 KB
• 参考文献：1.White JJ, Sillitoe RV. Development of the cerebellum: from gene expression patterns to circuit maps. Wiley Interdiscip Rev Dev Biol. 2013;2:149–64.CrossRef PubMed
  2.Hashimoto M, Mikoshiba K. Mediolateral compartmentalization of the cerebellum is determined on the “birth date” of Purkinje cells. J Neurosci. 2003;23:11342–51.PubMed
  3.Sudarov A, Turnbull RK, Kim EJ, Lebel-Potter M, Guillemot F, Joyner AL. Ascl1 genetics reveals insights into cerebellum local circuit assembly. J Neurosci. 2011;31:11055–69.PubMedCentral CrossRef PubMed
  4.Croci L, Chung SH, Masserdotti G, Gianola S, Bizzoca A, Gennarini G, Corradi A, Rossi F, Hawkes R, Consalez GG. A key role for the HLH transcription factor EBF2COE2, O/E-3 in Purkinje neuron migration and cerebellar cortical topography. Development. 2006;133:2719–29.
  5.Millen K, Hui C, Joyner A. A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum. Development. 1995;121:3935–45.PubMed
  6.Larouche M, Hawkes R. From clusters to stripes: the developmental origins of adult cerebellar compartmentation. Cerebellum. 2006;5:77–88.CrossRef PubMed
  7.Wassef M, Zanetta JP, Brehier A, Sotelo C. Transient biochemical compartmentalization of Purkinje cells during early cerebellar development. Dev Biol. 1985;111:129–37.CrossRef PubMed
  8.Brochu G, Maler L, Hawkes R. Zebrin II: a polypeptide antigen expressed selectively by Purkinje cells reveals compartments in rat and fish cerebellum. J Comp Neurol. 1990;291:538–52.CrossRef PubMed
  9.Marzban H, Chung S, Watanabe M, Hawkes R. Phospholipase Cbeta4 expression reveals the continuity of cerebellar topography through development. J Comp Neurol. 2007;502:857–71.CrossRef PubMed
  10.Fujita H, Morita N, Furuichi T, Sugihara I. Clustered fine compartmentalization of the mouse embryonic cerebellar cortex and its rearrangement into the postnatal striped configuration. J Neurosci. 2012;32:15688–703.CrossRef PubMed
  11.White JJ, Arancillo M, Stay TL, George-Jones NA, Levy SL, Heck DH, Sillitoe RV. Cerebellar zonal patterning relies on Purkinje cell neurotransmission. J Neurosci. 2014;34:8231–45.
  12.Sotelo C. Cellular and genetic regulation of the development of the cerebellar system. Prog Neurobiol. 2004;72:295–339.CrossRef PubMed
  13.Apps R, Hawkes R. Cerebellar cortical organization: a one-map hypothesis. Nat Rev Neurosci. 2009;10:670–81.CrossRef PubMed
  14.Sillitoe RV, Vogel MW, Joyner AL. Engrailed homeobox genes regulate establishment of the cerebellar afferent circuit map. J Neurosci. 2010;30:10015–24.PubMedCentral CrossRef PubMed
  15.Kalinovsky A, Boukhtouche F, Blazeski R, Bornmann C, Suzuki N, Mason CA, Scheiffele P. Development of axon-target specificity of ponto-cerebellar afferents. PLoS Biol. 2011;9:e1001013.
  16.Reeber SL, Gebre SA, Sillitoe RV. Fluorescence mapping of afferent topography in three dimensions. Brain Struct Funct. 2011;216:159–69.CrossRef PubMed
  17.White JJ, Sillitoe RV. Postnatal development of cerebellar zones revealed by neurofilament heavy chain protein expression. Front Neuroanat. 2013;7:9.PubMedCentral PubMed
  18.Goshgarian HG, Buttry JL. The pattern and extent of retrograde transsynaptic transport of WGA-Alexa 488 in the phrenic motor system is dependent upon the site of application. J Neurosci Methods. 2014;222:156–64.PubMedCentral CrossRef PubMed
  19.Takeda T, Maekawa K. Transient direct connection of vestibular mossy fibers to the vestibulocerebellar Purkinje cells in early postnatal development of kittens. Neuroscience. 1989;32:99–111.CrossRef PubMed
  20.Tolbert DL, Pittman T, Alisky JM, Clark BR. Chronic NMDA receptor blockade or muscimol inhibition of cerebellar cortical neuronal activity alters the development of spinocerebellar afferent topography. Dev Brain Res. 1994;80:268–74.CrossRef
  
• 作者单位：Roy V. Sillitoe (1)
  
  1. Department of Pathology and Immunology, Department of Neuroscience, Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute of Texas Children’s Hospital, 1250 Moursund Street, Suite 1325, Houston, TX, 77030, USA
  
• 刊物主题：Neurosciences; Neurology; Neurobiology;
• 出版者：Springer US
• ISSN：1473-4230

文摘

The cerebellum is organized into a map of zones that is manifested in various ways according to gene expression, anatomical connectivity, neuronal firing properties, behavioral specificity, and susceptibility to disease. At the center of every zone is the Purkinje cell, the principal cell type of the cerebellum and sole output of the cerebellar cortex. During development, Purkinje cells are thought to coordinate the zonal patterning of all other cell types. However, the morphogenetic mechanism that mediates the interaction between Purkinje cells and afferent fibers remains unclear. To address this problem in vivo, I took advantage of a rapid fluorescent-based transynaptic tracing approach to determine the nature of mossy fiber to Purkinje cell connectivity during early postnatal development, a period when the afferent map is assembling into clear-cut zonal circuits. By injecting WGA-Alexa 555 into the lower thoracic-upper lumber spinal cord, I found that spinocerebellar mossy fibers transynaptically transfer tracer into zones of Purkinje cells that are directly adjacent to the fibers. The traced Purkinje cell zones formed a zebrin-like pattern that was defined by the expression of neurofilament heavy chain (NFH), a marker of zones in the postnatal developing cerebellum. These results suggest that Purkinje cells generate the zonal circuit map by using molecular cues, neuronal activity, and synaptic contact. Keywords Spinocerebellar Zones Postnatal Circuitry Neural tracing Transynaptic