The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus

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• 作者：Rachel Wells (1)
  Martin Trick (1)
  Eleni Soumpourou (1)
  Leah Clissold (1) (2)
  Colin Morgan (1)
  Peter Werner (3)
  Carl Gibbard (3)
  Matthew Clarke (4)
  Richard Jennaway (5)
  Ian Bancroft (1) (6)
  
• 关键词：Desaturation ; Fatty acid biosynthesis ; Brassica ; Oleic acid ; Polyunsaturated fatty acids ; EMS
• 刊名：Molecular Breeding
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：33
• 期：2
• 页码：349-362
• 全文大小：338 KB
• 作者单位：Rachel Wells (1)
  Martin Trick (1)
  Eleni Soumpourou (1)
  Leah Clissold (1) (2)
  Colin Morgan (1)
  Peter Werner (3)
  Carl Gibbard (3)
  Matthew Clarke (4)
  Richard Jennaway (5)
  Ian Bancroft (1) (6)
  
  1. John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK
  2. The Genome Analysis Centre, Norwich Research Park, Norwich, NR4 7UH, UK
  3. KWS UK Ltd., 56 Church Street, Thriplow, Hertfordshire, SG8 7RE, UK
  4. Monsanto UK Ltd., PO Box 663, Cambridge, CB1 0LD, UK
  5. Saaten-Union UK Ltd., Rosalie Field Station, Bradley Road, Cowlinge, Newmarket, Suffolk, CB8 9HU, UK
  6. Department of Biology, University of York, Heslington, York, YO41 5DD, UK
  
• ISSN：1572-9788

文摘

Many important plant species have polyploidy in their recent ancestry, complicating inferences about the genetic basis of trait variation. Although the principal locus controlling the proportion of polyunsaturated fatty acids (PUFAs) in seeds of Arabidopsis thaliana is known (fatty acid desaturase 2; FAD2), commercial cultivars of a related crop, oilseed rape (Brassica napus), with very low PUFA content have yet to be developed. We showed that a cultivar of oilseed rape with lower than usual PUFA content has non-functional alleles at three of the four orthologous FAD2 loci. To explore the genetic basis further, we developed an ethyl methanesulphonate mutagenised population, JBnaCAB_E, and used it to identify lines that also carried mutations in the remaining functional copy. This confirmed the hypothesised basis of variation, resulting in an allelic series of mutant lines showing a spectrum of PUFA contents of seed oil. Several lines had PUFA content of ~6?% and oleic acid content of ~84?%, achieving a long-standing industry objective: very high oleic, very low PUFA rapeseed without the use of genetic modification technology. The population contains a high rate of mutations and represents an important resource for research in B. napus.