Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.)
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- 作者：Carmen Leida (1)
Claudio Moser (1)
Cristina Esteras (2)
Ronan Sulpice (3) (4)
John E Lunn (3)
Frank de Langen (5)
Antonio J Monforte (6)
Belen Pic贸 (2)
1. Research and Innovation Center ; Department Genomics and Biology of Fruit Crops ; Fondazione Edmund Mach (FEM) ; Via E. Mach 1 ; 38010 ; San Michele ; Italy
2. Institute for the Conservation and Breeding of Agricultural Biodiversity (COMAV-UPV) ; Universitat Polit猫cnica de Valencia ; Camino de Vera s/n ; 46022 ; Valencia ; Spain
3. Max-Planck-Institute of Molecular Plant Physiology ; Wissenschaftspark Golm ; Am M眉hlenberg 1 ; 14476 ; Potsdam ; Germany
4. Plant Systems Biology Research Laboratory ; Department of Botany and Plant Science ; Plant and AgriBiosciences Research Centre ; National University of Galway ; University Road ; Galway ; Ireland
5. HMCLAUSE (Business Unit of Limagrain) ; Station de Mas Saint Pierre ; La Galine ; 13210 ; Saint-R茅my-de-Provence ; France
6. Instituto de Biolog铆a Molecular y Celular de Plantas (IBMCP) ; Universitat Polit猫cnica de Val猫ncia (UPV)-Consejo Superior de Investigaciones Cient铆ficas (CSIC) ; Ciudad Polit茅cnica de la Innovaci贸n (CPI) ; Ed. 8E ; C/Ingeniero Fausto Elio s/n ; 46022 ; Valencia ; Spain - 关键词：Melon ; Climacteric ripening ; Sugar ; Germplasm collection
- 刊名：BMC Genetics
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：16
- 期：1
- 全文大小：1,529 KB
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- 出版者：BioMed Central
- ISSN：1471-2156
文摘
Background A collection of 175 melon (Cucumis melo L.) accessions (including wild relatives, feral types, landraces, breeding lines and commercial cultivars) from 50 countries was selected to study the phenotypic variability for ripening behavior and sugar accumulation. The variability of single nucleotide polymorphisms (SNPs) at 53 selected candidate genes involved in sugar accumulation and fruit ripening processes was studied, as well as their association with phenotypic variation of related traits. Results The collection showed a strong genetic structure, defining seven groups plus a number of accessions that could not be associated to any of the groups (admixture), which fitted well with the botanical classification of melon varieties. The variability in candidate genes for ethylene, cell wall and sugar-related traits was high and similar to SNPs located in reference genes. Variability at ripening candidate genes had an important weight on the genetic stratification of melon germplasm, indicating that traditional farmers might have selected for ripening traits during cultivar diversification. A strong relationship was also found between the genetic structure and phenotypic diversity, which could hamper genetic association studies. Accessions belonging to the ameri group are the most appropriate for association analysis given the high phenotypic and molecular diversity within the group, and lack of genetic structure. The most remarkable association was found between sugar content and SNPs in LG III, where a hotspot of sugar content QTLs has previously been defined. By studying the differences in allelic variation of SNPs within horticultural groups with specific phenotypic features, we also detected differential variation in sugar-related candidates located in LGIX and LGX, and in ripening-related candidates located in LGII and X, all in regions with previously mapped QTLs for the corresponding traits. Conclusions In the current study we have found an important variability at both the phenotypic and candidate gene levels for ripening behavior and sugar accumulation in melon fruit. By combination of differences in allelic diversity and association analysis, we have identified several candidate genes that may be involved in the melon phenotypic diversity.