Molecular cloning and expression analysis of four turmeric MAP kinase genes in response to abiotic stresses and phytohormones

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• 作者：S. Nanda (1)
  S. Nayak (1)
  R. K. Joshi (1)
  
• 关键词：abscisic acid ; ClMPKs ; chromium ; cold ; Curcuma longa ; methyl jasmonate ; RT ; qPCR ; salinity ; water stress
• 刊名：Biologia Plantarum
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：58
• 期：3
• 页码：479-490
• 全文大小：2,247 KB
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• 作者单位：S. Nanda (1)
  S. Nayak (1)
  R. K. Joshi (1)
  
  1. Centre of Biotechnology, Siksha O. Anusandhan University, Bhubaneswar, 751003, India
  
• ISSN：1573-8264

文摘

Plant mitogen activated protein kinase (MAPK) cascades comprise a complex network playing a major role in regulating extracellular stimuli as well as developmental processes. The present study involves cloning four MAPKs (ClMPK1, 3, 4 and 5) from Curcuma longa. All four ClMPKs have fully canonical motifs of MAPK and each is represented by a single copy in the turmeric genome. The analysis of exon-intron junctions revealed conserved nature of ClMPKs across different plant groups. The RT-qPCR analysis showed their expression in mature plant tissues. The transcript analysis using the RT-qPCR shows that the four ClMPKs were differentially regulated by cold, salinity, and drought stresses. ClMPK4 showed a significant upregulation in the presence of NaCl, polyethylene glycol, and mannitol. The time-course expression analysis revealed a marked accumulation of ClMPK1 and ClMPK4 transcripts after mechanical wounding or applications of abscisic acid, H2O2, methyl jasmonate, and salicylic acid. ClMPK5 showed a unique and pronounced expression in response to hexavalent chromium (CrVI).