cDNA sequence and protein bioinformatics analyses of MSTN in African catfish (Clarias gariepinus)

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• 作者：Poonmanee Kanjanaworakul ; Orathai Sawatdichaikul…
• 关键词：Clarias gariepinus ; Myostatin ; cDNA cloning ; Protein modeling ; 3D structure
• 刊名：Molecular Biology Reports
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：43
• 期：4
• 页码：283-293
• 全文大小：1,060 KB
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• 作者单位：Poonmanee Kanjanaworakul (1) (2)
  Orathai Sawatdichaikul (3)
  Supawadee Poompuang (4)
  
  1. Center for Agricultural Biotechnology, Kasetsart University, Nakorn Pathom, 73140, Thailand
  2. Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand
  3. Department of Nutrition and Health, Institute of Food Research and Product Development, Kasetsart University, Bangkok, 10900, Thailand
  4. Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

Myostatin, also known as growth differentiation factor 8, has been identified as a potent negative regulator of skeletal muscle growth. The purpose of this study was to characterize and predict function of the myostatin gene of the African catfish (Cg-MSTN). Expression of Cg-MSTN was determined at three growth stages to establish the relationship between the levels of MSTN transcript and skeletal muscle growth. The partial cDNA sequence of Cg-MSTN was cloned by using published information from its congener walking catfish (Cm-MSTN). The Cg-MSTN was 1194 bp in length encoding a protein of 397 amino acids. The deduced MSTN sequence exhibited key functional sites similar to those of other members of the TGF-β superfamily, especially, the proteolytic processing site (RXXR motif) and nine conserved cysteines at the C-terminal. Expression of MSTN appeared to be correlated with muscle development and growth of African catfish. Protein bioinformatics revealed that the primary sequence of Cg-MSTN shared 98 % sequence identity with that of walking catfish Cm-MSTN with only two different residues, \(L_{Cm}^{191} /V_{Cg}^{192}\). and \(S_{Cm}^{289} /P_{Cg}^{290}\). The proposed model of Cg-MSTN revealed the key point mutation \(S_{Cm}^{289} /P_{Cg}^{290}\) causing a 7.35 Å shorter distance between the N- and C-lobes and an approximately 11° narrow angle than those of Cm-MSTN. The substitution of a proline residue near the proteolytic processing site which altered the structure of myostatin may play a critical role in reducing proteolytic activity of this protein in African catfish.