Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies
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- 作者：Laure-Hélène Ouisse ; Laetitia Gautreau-Rolland ; Marie-Claire Devilder…
- 关键词：Humanized rats ; Human antibodies ; Tetramers ; pMHC ; Cytofluorimetry
- 刊名：BMC Biotechnology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：3272KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Applied Microbiology; Biotechnology; Biochemical Engineering; Genetic Engineering; Plant Breeding/Biotechnology; Biomedical Engineering/Biotechnology;
- 出版者：BioMed Central
- ISSN：1472-6750
- 卷排序：17
文摘
BackgroundThere is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied a strategy for the isolation of antigen-specific B cells using tetramerized proteins and single-cell sorting followed by reconstruction of human mAbs by RT-PCR and expression cloning.