Upregulation of miR-494 Inhibits Cell Growth and Invasion and Induces Cell Apoptosis by Targeting Cleft Lip and Palate Transmembrane 1-Like in Esophageal Squamous Cell Carcinoma

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• 作者：Ren Zhang ; Xiaonan Chen ; Shengjie Zhang ; Xueyan Zhang…
• 关键词：miR ; 494 ; CLPTM1L ; Esophageal squamous cell carcinoma ; Proliferation ; Invasion ; Apoptosis
• 刊名：Digestive Diseases and Sciences
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：60
• 期：5
• 页码：1247-1255
• 全文大小：2,654 KB
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• 作者单位：Ren Zhang (1)
  Xiaonan Chen (1)
  Shengjie Zhang (1)
  Xueyan Zhang (1)
  Tong Li (1)
  Zhicai Liu (2)
  Jinwu Wang (2)
  Wenqiao Zang (1)
  Yuanyuan Wang (1)
  Yuwen Du (1)
  Guoqiang Zhao (1)
  
  1. College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, Zhengzhou, 450001, Henan, China
  2. Oncology Department, The Tumor Hospital of Linzhou City, Linzhou, 456500, Henan, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Gastroenterology
  Hepatology
  Oncology
  Transplant Surgery
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-2568

文摘

Background Potential target genes of microRNA (miR)-494 have been reported in many types of cancers. However, the role of miR-494 in esophageal squamous cell carcinoma (ESCC) remains unknown. Aim This study focused on the expression and biological function of miR-494 in ESCC. Methods Using bioinformatics analyses, we found that cleft lip and palate transmembrane 1-like (CLPTM1L) was a potential target of miR-494. We performed quantitative real-time (qRT) PCR assays in 37 ESCC tumor tissues to determine the expression of miR-494 and CLPTM1L mRNA, and we analyzed the correlation between both of these factors and clinical characteristics. The cell counting kit-8 and colony formation assays were used to evaluate the effects of miR-494 expression on the proliferation of ESCC cells. The transwell migration assay and flow cytometric apoptosis assay were performed to study the influence of miR-494 on the invasion and apoptosis of ESCC cells. Western blotting, luciferase assays, and CLPTM1L knockdown experiments were used to determine whether CLPTM1L was a target of miR-494. Results The qRT-PCR assays showed significant downregulation of miR-494 (P?<?0.05) and upregulation of CLPTM1L mRNA (P?<?0.05), both of which were significantly associated with lymph node metastases (P?<?0.05). High expression of miR-494 inhibited cell proliferation and invasion and promoted cell apoptosis (P?<?0.05). The results also showed that CLPTM1L was a target of miR-494. Conclusion These results show that the expression of miR-494, which can regulate cell growth, invasion and apoptosis of ESCC cells by targeting CLPTM1L, is downregulated in ESCC tumor tissues. The miR-494–CLPTM1L pathway could be further exploited to develop a new approach to treat ESCC.