Analysis of circulating hem-endothelial marker RNA levels in preterm infants

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• 作者：Tzipora Strauss (1)
  Sally Metsuyanim (2)
  Itai Pessach (3)
  Irit Shuchan-Eisen (1)
  Jacob Kuint (1)
  Benjamin Dekel (2) (4)
  
• 刊名：BMC Pediatrics
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：9
• 期：1
• 全文大小：541KB
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  37. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2431/9/42/prepub
  
• 作者单位：Tzipora Strauss (1)
  Sally Metsuyanim (2)
  Itai Pessach (3)
  Irit Shuchan-Eisen (1)
  Jacob Kuint (1)
  Benjamin Dekel (2) (4)
  
  1. Neonatal Department, Chaim Sheba Medical Center, Tel Aviv University, Tel Aviv, Israel
  2. Pediatric Stem Cell Research Institute Chaim Sheba Medical Center, Tel Aviv University, Tel Aviv, Israel
  3. Division of Immunology, Children's Hospital, Harvard Medical School, Boston, USA
  4. Department of Pediatrics, Chaim Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
  

文摘

Background Circulating endothelial cells may serve as novel markers of angiogenesis. These include a subset of hem-endothelial progenitor cells that play a vital role in vascular growth and repair. The presence and clinical implications of circulating RNA levels as an expression for hematopoietic and endothelial-specific markers have not been previously evaluated in preterm infants. This study aims to determine circulating RNA levels of hem-endothelial marker genes in peripheral blood of preterm infants and begin to correlate these findings with prenatal complications. Methods Peripheral blood samples from seventeen preterm neonates were analyzed at three consecutive post-delivery time points (day 3-, 10-5 and 30). Using quantitative reverse transcription-polymerase chain reaction we studied the expression patterns of previously established hem-endothelial-specific progenitor-associated genes (AC133, Tie-2, Flk-1 (VEGFR2) and Scl/Tal1) in association with characteristics of prematurity and preterm morbidity. Results Circulating Tie-2 and SCL/Tal1 RNA levels displayed an inverse correlation to gestational age (GA). We observed significantly elevated Tie-2 levels in preterm infants born to mothers with amnionitis, and in infants with sustained brain echogenicity on brain sonography. Other markers showed similar expression patterns yet we could not demonstrate statistically significant correlations. Conclusion These preliminary findings suggest that circulating RNA levels especially Tie2 and SCL decline with maturation and might relate to some preterm complication. Further prospective follow up of larger cohorts are required to establish this association.