Immunophenotyping and spatio-temporal distribution of aortic cell clusters in the bovine embryo
详细信息 查看全文
- 作者：M. Kritzenberger (1)
K.-H. Wrobel (1) - 刊名：Brain Structure and Function
- 出版年：2006
- 出版时间：November 2006
- 年：2006
- 卷：211
- 期：6
- 页码：739-755
- 全文大小：1379KB
- 参考文献：1. Bernex F, De Sepulveda P, Kress C, Elbaz C, Delouis C, Panthier J (1996) Spatial and temporal patterns of c-kit-expressing cells in WlacZ/+ and WlacZ/WlacZ mouse embryos. Development 122:3021-033
2. Brachtendorf G, Kuhn A, Samulowitz U, Knorr R, Gustafsson E, Potocnik AJ, F?ssler R, Vestweber D (2001) Early expression of endomucin on endothelium of the mouse embryo and on putative hematopoietic clusters in the dorsal aorta. Dev Dyn 222:410-19 CrossRef
3. de Bruijn MFTR, Speck NA, Peeters MCE, Dzierzak E (2000) Definitive hematopoietic stem cells first develop within the major arterial regions of the mouse embryo. EMBO J 19:2465-474 CrossRef
4. de Bruijn MFTR, Ma X, Robin C, Ottersbach K, Sanchez M-J, Dzierzak E (2002) Hematopoietic stem cells localize to the endothelial cell layer in the midgestation mouse aorta. Immunity 16:673-83 CrossRef
5. Charbord P, Oostendorp R, Pang W, Hérault O, Noel F, Tsuji T, Dzierzak E, Péault B (2002) Comparative study of stromal cell lines derived from embryonic, fetal, and postnatal mouse blood-forming tissues. Exp Hematol 30:1202-210 CrossRef
6. Coulombel L, Auffray I, Gaugler M-H, Rosemblatt M (1997) Expression and function of integrins on hematopoietic progenitor cells. Acta Haematol 97:13-1 CrossRef
7. Cumano A, Godin I (2001) Pluripotent hematopoietic stem cell development during embryogenesis. Curr Opin Immunol 13:166-71 CrossRef
8. Dieterlen-Lièvre F, Pardanaud L, Caprioli A, Jaffredo T (2001) Non-yolk sac hematopoietic stem cells: the avian paradigm. In: Zon LI (eds) Hematopoiesis a developmental approach. Oxford University Press, New York, pp 201-08
9. Dzierzak E, Medvinsky A (1995) Mouse embryonic hematopoiesis. TIG 11:359-66
10. Dzierzak E, Oostendorp R (2001) Hematopoietic stem cell development in mammals. In: Zon LI (ed) Hematopoiesis a developmental approach. Oxford University Press, New York, pp 209-17
11. Dzierzak E, Medvinsky A, de Bruijn M (1998) Qualitative and quantitative aspects of haematopoietic cell development in the mammalian embryo. Immunol Today 19:228-36 CrossRef
12. Emmel VE (1916) The cell clusters in the dorsal aorta of mammalian embryos. Am J Anat 19:401-21 CrossRef
13. Fraser ST, Ogawa M, Yokomizo T, Ito Y, Nishikawa S, Nishikawa SI (2003) Putative intermediate precursors between hematogenic endothelial cells and blood cells in the developing embryo. Dev Growth Differ 45:63-5 CrossRef
14. Galloway JL, Zon LI (2003) Ontogeny of hematopoiesis: examining the emergence of hematopoietic cells in the vertebrate embryo. Curr Top Dev Biol 53:139-58 CrossRef
15. Garcia-Porrero JA, Godin IE, Dieterlen-Lièvre F (1995) Potential intraembryonic hemogenic sites at pre-liver stages in the mouse. Anat Embryol 192:425-35 CrossRef
16. Garcia-Porrero JA, Manaia A, Jimeno J, Lasky LL, Dieterlen-Lièvre F, Godin IE (1998) Antigenic profiles of endothelial and hemopoietic lineages in murine intraembryonic hemogenic sites. Dev Comp Immunol 22:303-19 CrossRef
17. Hara T, Nakano Y, Tanaka M, Tamura K, Sekiguchi T, Minehata K, Copeland NG, Jenkins NA, Okabe M, Kogo H, Mokouyama Y, Miyajima A (1999) Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta–gonad–mesonephros region. Immunity 11:567-78 CrossRef
18. Harvey K, Dzierzak E (2004) Cell–cell contact and anatomical compatibility in stromal cell-mediated HSC support during development. Stem Cells 22:253-58 CrossRef
19. Hsu SM, Raine L, Fanger H (1981) Use of avidin–biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577-80
20. Huyhn A, Dommergues M, Izac B, Croisille L, Katz A, Vainchenker W, Coulombel L (1995) Characterization of hematopoietic progenitors from human yolk sac and embryos. Blood 86:4475-583
21. Jaffredo T, Gautier R, Eichmann A, Dieterlen-Lièvre F (1998) Intraaortic hemopoietic cells are derived from endothelial cells during ontogeny. Development 125:4575-583
22. Jordan HE (1917) Aortic cell clusters in vertebrate embryos. Proc Natl Acad Sci USA 3:149-56 CrossRef
23. Karnovsky MJ (1965) A formaldehyde–glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137A-38A
24. Kemmer M, Wrobel KH (2004) The role of the mesonephros as transient haematopoietic organ in bovine embryos. Ann Anat 186(Suppl.):243
25. Kritzenberger M, Wrobel K-H (2004) Histochemical in situ identification of bovine embryonic blood cells reveals differences to the adult haematopoietic system and suggests a close relationship between haematopoietic stem cells and primordial germ cells. Histochem Cell Biol 121:273-89 CrossRef
26. Kujat R, Rose C, Wrobel KH (1993) The innervation of the bovine ductus deferens: comparison of a modified acetylcholinesterase-reaction with immunoreactivities of cholinacetyl transferase and panneuronal markers. Histochemistry 99:231-39 CrossRef
27. Labastie MC, Cortés F, Roméo PH, Dulac C, Péault B (1998) Molecular identity of hematopoietic precursor cells emerging in the human embryo. Blood 92:3624-635
28. Ling K-W, Ottersbach K, van Hamburg JP, Oziemlak A, Tsai F-Y, Orkin SH, Ploemacher R, Hendriks RW, Dzierzak E (2004) GATA-2 plays two functionally distinct roles during the ontogeny of hematopoietic stem cells. J Exp Med 200:871-82 CrossRef
29. Marshall C (2001) The embryonic origins of human haematopoiesis. Br J Haematol 112:838-50 CrossRef
30. Marshall CJ, Moore RL, Thorogood P, Brickell PM, Kinnon C, Trasher AJ (1999) Detailed characterization of the human aorta–gonad–mesonephros region reveals morphological polarity resembling a hematopoietic stromal layer. Dev Dyn 215:139-47 CrossRef
31. Medvinsky A, Dzierzak E (1996) Definitive hematopoiesis is autonomously initiated by the AGM region. Cell 86:897-06 CrossRef
32. Medvinsky AL, Gan OI, Semenova ML, Samoylina NL (1996) Development of day-8 colony-forming unit-spleen hematopoietic progenitors during early murine embryogenesis: spatial and temporal mapping. Blood 87:557-66
33. Milroy AM (1985) A method for eliminating unnecessary serial thin sectioning for electron microscopy. J Electron Microsc Tech 2:267-68 CrossRef
34. North T, Gu TL, Stacy T, Wang Q, Howard L, Binder M, Marin-Padilla M, Speck N (1999) Cbfa2 is required for the formation of intraaortic hematopoietic clusters. Development 126:2563-575
35. North T, de Bruijn MFTR, Stacy T, Talebian L, Lind E, Robin C, Binder M, Dzierzak E (2002) Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. Immunity 16:661-72 CrossRef
36. Olah I, Medgyes J, Glick B (1988) Origin of aortic cell clusters in the chicken embryo. Anat Rec 222:60-8 CrossRef
37. Oostendorp RAJ, Harvey KN, Kusadasi N, de Bruijn MFTR, Saris C, Ploemacher RE, Medvinsky AL, Dzierzak EA (2002) Stromal cell lines from mouse aorta–gonads–mesonephros subregion are potent supporters of hematopoietic stem cell activity. Blood 2002:1183-189 CrossRef
38. Petrenko O, Beavis A, Klaine M, Kittappa R, Godin I, Lemischka IR (1999) The molecular characterization of the fetal stem cell marker AA4. Immunity 10:691-00 CrossRef
39. Reynolds EG (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208-12 CrossRef
40. Richardson KC, Jarett L, Finke HE (1960) Embedding in epoxy resin for ultrathin sectioning in electron microscopy. Stain Technol 35:313-23
41. Ruoslahti E (1988) Fibronectin and its receptors. Annu Rev Biochem 57:375-13 CrossRef
42. Sanchez MJ, Holmes A, Miles C, Dzierzak E (1996) Characterization of the first definitive hematopoietic stem cells in the AGM and liver of the mouse embryo. Immunity 5:513-25 CrossRef
43. Schabtach E, Parkening TA (1974) A method for sequential high resolution light and electron microscopy of selected areas of the same material. J Cell Biol 61:261-64 CrossRef
44. Shalaby F, Ho J, Stanford WL, Fischer KD, Schuh AC, Schwartz L, Bernstein A, Rossant J (1997) A requirement for flk1 in primitive and definitive hematopoiesis and vasculogenesis. Cell 89:981-90 CrossRef
45. Smith RA, Glomski CA (1982) “Hemogenic endothelium-of the embryonic aorta: does it exist? Dev Comp Immunol 6:359-68 CrossRef
46. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31-3 CrossRef
47. Sugiyama D, Ogawa M, Hirose I, Jaffredo T, Arai K, Tsuji K (2003) Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage. Blood 101:4733-738 CrossRef
48. Tavian M, Coulombel L, Luton D, Clemente HS, Dieterlen-Lièvre F, Péault B (1996) Aorta-associated CD34+ hematopoietic cells in the early human embryo. Blood 87:67-2
49. Tavian M, Hallais MF, Péault B (1999) Emergence of intraembryonic hematopoietic precursors in the pre-liver human embryo. Development 126:793-03
50. Watt SM, Butler LH, Tavian M, Bühring HJ, Rappold I, Simmons PJ, Zannettino ACW, Buck D, Fuchs A, Doyonnas R, Chan JYH, Levesque JP, Péault B, Roxanis J (2000) Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny but display distinct distribution patterns in adult hematopoietic and nonhematopoietic tissues. Blood 95:3113-124
51. Wood HB, May G, Healy L, Enver T, Moriss-Kay GM (1997) CD34 expression patterns during early mouse development are related to modes of blood vessel formation and reveal additional sites of hematopoiesis. Blood 90:2300-311
52. Wrobel KH, Sü? F (1998) Identification and temporospatial distribution of bovine primordial germ cells prior to gonadal sexual differentiation. Anat Embryol 197:451-67 CrossRef
53. Yoder MC, Williams DA (1995) Matrix molecule interactions with hematopoietic stem cells. Exp Hematol 23:961-67
54. Yoshida H, Takakura N, Hirashima M, Kataoka H, Tsuchida K, Nishikawa S, Nishikawa S (1998) Hematopoietic tissues, as a playground of receptor tyrosine kinases of the PDGF-receptor family. Dev Comp Immunol 22:2915-923 - 作者单位：M. Kritzenberger (1)
K.-H. Wrobel (1)
1. Institute of Anatomy, University of Regensburg, 93040, Regensburg, Germany
文摘
In the present study the temporal and spatial appearance of aortic cell clusters in bovine embryos is described. Aorta-associated c-kit-positive cell clusters can be observed first in 23?days post inseminationem (dpi) bovine embryos and disappear after 34?dpi. For the first time, it was shown that the immunophenotype of these aortic cluster cells changes during embryonic development. Aortic cell clusters are c-kit+/CD45-/STA? when they are first detected in the 23?dpi embryo, and acquire a c-kit+/CD45+/STA?phenotype in 27-9 embryos and a c-kit+/CD45+/STA+?immunophenotype in 32-4-day-old specimens. Cell clusters are most prominent in the vicinity of lateral and ventral aortic branches, but rare in omphalomesenteric arteries and absent in Aa. umbilicales. Free c-kit-positive cells in an intravasal position are common, suggesting separation from the clusters in order to colonize subsequent hematopoietic organs, i.e., the liver and the mesonephros. Transmission electron microscopic analysis reveals the existence of primitive desmosomes between the clusters cells and adjacent endothelial cells as well as a fine basal lamina as a demarcation between the cluster cells and underlying mesenchymal cells. Material resembling extracellular matrix is found in large vacuoles in cluster cells of 23?dpi embryos. Immunocytochemistry reveals an intense accumulation of heparan sulfate proteoglycan and collagen IV in the aortic wall at the sites where cell clusters are attached. These observations suggest that the hematopoietic cell clusters induce the formation of a specific microenvironment within the aortic wall.