Aorta transplantation in young apolipoprotein E-deficient mice: Possible model for studies on regression of atherosclerotic lesions?
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- 作者：Zbyněk Tonar (1) (2) (3)
Dagmar Bobková (1)
Kirsti Witter (4)
Vít Martin Matějka (2)
Jana Havlí?ková (5)
Věra Lánská (6)
Rudolf Poledne (1) - 关键词：Heterotopic transplantation ; Quantitative histology ; Stereology
- 刊名：Central European Journal of Medicine
- 出版年：2010
- 出版时间：June 2010
- 年：2010
- 卷：5
- 期：3
- 页码：280-291
- 全文大小：1863KB
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Dagmar Bobková (1)
Kirsti Witter (4)
Vít Martin Matějka (2)
Jana Havlí?ková (5)
Věra Lánská (6)
Rudolf Poledne (1)
1. Laboratory for Atherosclerosis Research, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21, Prague, Czech Republic
2. Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarská 48, 301 66, Pilsen, Czech Republic
3. Department of Mechanics, Faculty of Applied Sciences, University of West Bohemia, Univerzitní 22, 306 14, Pilsen, Czech Republic
4. Institute of Histology and Embryology, Department of Pathobiology, University of Veterinary Medicine, Veterin?rplatz 1, A-1210, Vienna, Austria
5. Laboratory of Pathophysiology of Cardiovascular System, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21, Prague, Czech Republic
6. Statistics Unit, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21, Prague, Czech Republic
文摘
Syngeneic transplantation of murine aorta segments with advanced atherosclerotic lesions in defined recipients is a valuable model for regression studies. To date, this model has not been used to study the regression of initial atherosclerotic lesions. The aim of this study was to evaluate a microsurgical technique of syngeneic heterotopic transplantation of the thoracic aorta of young apolipoprotein E-deficient (ApoE-/-) mice to the abdominal aorta of wild-type recipients. Stereological quantification methods were tested in order to assess changes in structure and volume of the aortic wall including the involvement of immune cells in changes of the atherosclerotic lesions. The animals were euthanised one month after surgery and histological analysis including stereological quantification of changes in both the grafts and adjacent aorta segments was performed. The overall survival rate of the recipients was 62.5%. No regression of initial atherosclerotic lesion was achieved and neointima formation and elastin degradation prevailed in all transplanted specimens. The volume of the arteriosclerotic lesions was higher (p<0.001) and elastin length density was lower (p<0.001) in transplanted ApoE-/- samples as compared to adjacent segments. In transplanted grafts, T- and B-lymphocytes, macrophages and neutrophilic granulocytes formed non-random clusters within the vessel wall and they were colocalised with the sutures. The reproducibility of the promising regression model was derogated in young mice by the striking dependence of the results upon the operation technique. Stereological assessment has proven to be accurate, correct and reproducible; it has provided us with robust quantitative estimates, which can be achieved with a reasonable effort.