Development of a genetic transformation system for distylous Turnera joelii (Passifloraceae) and characterization of a self-compatible mutant

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• 作者：Paul D. J. Chafe (1)
  Timothy Lee (1) (2)
  Joel S. Shore (1)
  
  1. Department of Biology ; York University ; 4700 Keele Street ; Toronto ; ON ; M3J 1P3 ; Canada
  2. Department of Molecular Genetics ; University of Toronto ; 1 King鈥檚 College Circle ; Toronto ; ON ; M5S 1A8 ; Canada
  
• 关键词：Distyly ; Genetic transformation ; Self ; compatible mutant ; Transgenic ; Turnera
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：120
• 期：2
• 页码：507-517
• 全文大小：404 KB
• 参考文献：1. Alcaraz-Mel茅ndez L, Real-Cos铆o S, Bashar Y (1994) Domestication of micropropagated plants of the spice damiana ( / Turnera diffusa). Plant Cell Rep 13:679鈥?82 CrossRef
  2. Alcaraz-Mel茅ndez L, Real-Cos铆o S, Robert ML (2002) Morphological comparison of damiana ( / Turnera diffusa, Willd.) regenerated in vitro from leaves cultured in solidified medium and liquid cultures. Sci Hortic 96:293鈥?01 CrossRef
  3. Athanasiou A, Khosravi D, Tamari F, Shore JS (2003) Characterization and localization of short-specific polygalacturonase in distylous / Turnera subulata (Turneraceae). Am J Bot 90:675鈥?82 CrossRef
  4. Barrett SCH, Shore JS (2008) New insights on heterostyly: Comparative biology, ecology and genetics. In: Franklin-Tong VE (ed) Self-incompatibility in flowering plants: evolution, diversity and mechanisms. Springer-verlag, Berlin, pp 3鈥?2 CrossRef
  5. Bateson W, Gregory RP (1905) On the inheritance of heterostylism in / Primula. Proc R Soc Lond B 76:581鈥?86 CrossRef
  6. Clough SJ, Bent AF (1998) Floral dip: a simplified method for / Agrobacterium-mediated transformation of / Arabidopsis thaliana. Plant J 16:735鈥?43 CrossRef
  7. Darwin C (1877) The different forms of flowers on plants of the same species. John Murray, London CrossRef
  8. De Buck S, Jacobs A, Van Montagu M, Depicker A (1999) The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. Plant J 20:295鈥?04 CrossRef
  9. Deroles SC, Gardner RC (1988) Analysis of the T-DNA structure in a large number of transgenic petunias generated by / Arobacterium-mediated transformation. Plant Mol Biol 11:365鈥?77 27393" target="_blank" title="It opens in new window">CrossRef
  10. D铆az-Rondero AJ, Alcaraz-Mel茅ndez L (1987) Callus induction and plantlet regeneration in damiana ( / Turnera diffusa, Willd.). Plant Cell, Tissue Org Cult 10:39鈥?5 CrossRef
  11. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11鈥?5
  12. Dutta I, Kottackal M, Tumimbang E, Tajima H, Zaid A, Blumwald E (2013) Sonication-assisted efficient / Agrobacterium-mediated genetic transformation of the multipurpose woody desert shrub / Leptadenia / pyrotechnica. Plant Cell Tissue Org Cult 112:289鈥?01 CrossRef
  13. Filipecki M, Malepszy M (2006) Unintended consequences of plant transformation. J Appl Genet 47:277鈥?86 CrossRef
  14. Gilmartin PM, Li J (2010) Delineation of the / S-locus in / Turnera subulata鈥攈oming in on heterostyly. Heredity 105:161鈥?62 CrossRef
  15. Hanahan D (1985) DNA cloning, a practical approach. In: Glover DM (ed) DNA cloning, a practical approach, vol 1. IRL Press, Oxford, pp 109鈥?35
  16. Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible binary Ti vector for / Agrobacterium-mediated plant transformation. Plant Mol Biol 42:819鈥?32 CrossRef
  17. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice ( / Oryza sativa L.) mediated by / Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271鈥?82 271.x" target="_blank" title="It opens in new window">CrossRef
  18. Khosravi D, Yang E, Siu K, Shore JS (2004) High level of 伪-dioxygenase in short styles of distylous / Turnera species. Int J Plant Sci 165:995鈥?006 CrossRef
  19. Kumar SV, Misquitta RW, Reddy VS, Rao BJ, Rajam MV (2004) Genetic transformation of the green alga- / Chlamydomonas reinhardtii by / Agrobacterium / tumefaciens. Plant Sci 166:731鈥?38 CrossRef
  20. Labonne JDJ, Shore JS (2011) Positional cloning of the / s haplotype determining the floral and incompatibility phenotype of the long-styled morph of distylous / Turnera subulata. Mol Genet Genomics 285:101鈥?11 CrossRef
  21. Labonne JDJ, Goultiaeva A, Shore JS (2009) High-resolution mapping of the / S-locus in / Turnera leads to the discovery of three genes tightly associated with the S-alleles. Mol Genet Genomics 281:673鈥?85 CrossRef
  22. Labonne JDJ, Tamari F, Shore JS (2010) Characterization of X-ray-generated floral mutants carrying deletions at the / S-locus of distylous / Turnera subulata. Heredity 105:235鈥?43 CrossRef
  23. Larkin PJ, Scowcroft WR (1981) Somaclonal variation鈥攁 novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197鈥?14 CrossRef
  24. Lavia G, Fernandez A, Marquez G (1994) Chromosome doubling in / Turnera ulmifolia (Turneraceae) induced by regeneration of plants from in vitro cultured leaf explants. Plant Syst Evol 192:41鈥?8 CrossRef
  25. Lazo GR, Stein PA, Ludwig RA (1991) A DNA transformation-competent / Arabidopsis genomic library in / Agrobacterium. Biotechnology 9:963鈥?67 CrossRef
  26. Lewis D, Jones DA (1992) The genetics of heterostyly. In: Barrett SCH (ed) Evolution and Function of Heterostyly. Springer-verlag, New York, pp 129鈥?50 CrossRef
  27. Li J, Webster MA, Smith MC, Gilmartin PM (2011) Floral hetermorphy in / Primula vulgaris: progress towards isolation and characterization of the / S locus. Ann Bot 108:715鈥?26 CrossRef
  28. Manders G, Otoni WC, d鈥橴tra Vaz FB, Blackhall NW, Power JB, Davey MR (1994) Transformation of passionfruit ( / Passiflora edulis fv / flavicarpa Degener.) using / Agrobacterium tumefaciens. Plant Cell Rep 13:697鈥?02 27" target="_blank" title="It opens in new window">CrossRef
  29. McCubbin AG, Lee C, Hetrick A (2006) Identification of genes showing differential expression between morphs in developing flowers of / Primula vulgaris. Sex Plant Reprod 19:63鈥?2 CrossRef
  30. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473鈥?97 CrossRef
  31. Nagano M, Aii J, Campbell C, Adachi T, Kawasaki S (2005) Construction of a BAC library for the investigation of the S locus in Buckwheat. Fagopyrum 22:13鈥?0
  32. Phillips RL, Kaeppler SM, Olhoft P (1994) Genetic instability of plant tissue cultures: breakdown of normal controls. Proc Natl Acad Sci USA 91:5222鈥?226 CrossRef
  33. SAS Institute Inc. (2013) SAS 9.4 help and documentation. SAS Institute Inc., Cary
  34. Shen W-J, Forde BG (1989) Efficient transformation of / Agrobacterium spp. by high voltage electroporation. Nucleic Acid Res 17:8385 CrossRef
  35. Shore JS (1991) Chromosomal evidence for autotetraploidy in the / Turnera ulmifolia complex (Turneraceae). Can J Bot 69:1302鈥?308 CrossRef
  36. Shore JS, Barrett SCH (1985) The genetics of distyly and homostyly in / Turnera ulmifolia L. (Turneraceae). Heredity 55:167鈥?74 CrossRef
  37. Snow R (1963) Alcoholic hydrochloric acid-carmine as a stain for chromosomes in squash preparations. Stain Technol 38:9鈥?3
  38. St枚ger E, Fink C, Pfosser M, Heberle-Bors E (1995) Plant transformation by particle bombardment of embryonic pollen. Plant Cell Rep 14:273鈥?78 27" target="_blank" title="It opens in new window">CrossRef
  39. Tamari F, Athanasiou A, Shore JS (2001) Pollen tube growth and inhibition in distylous and homostylous / Turnera and / Piriqueta (Turneraceae). Can J Bot 79:578鈥?91
  40. Ushijima K, Nakano R, Bando M, Shigenzane Y, Ikeda K, Namba Y, Kume S, Kitabata T, Mori H, Kubo Y (2012) Isolation of the floral morph-related genes in heterostylous flax ( / Linum grandiflorum): the genetic polymorphism and the transcriptional and post-transcriptional regulations of the / S locus. Plant J 69:317鈥?31 CrossRef
  41. Wang YH (2008) How effective is T-DNA insertional mutagenesis in / Arabidopsis? J Biochem Tech 1:11鈥?0
  42. Winkler LM, Quoirin M (2002) Organogenesis and genetic transformation of yellow passion fruit ( / Passiflora edulis f. / flavicarpa Deg.) with the gene / CMe- / ACO1 and / nptII via / Agrobacterium tumefaciens. Acta Hortic 632:31鈥?0
  43. Yasui Y, Mori M, Aii J, Abe T, Matsumoto D, Sato S, Hayashi Y, Ohnishi O, Ota T (2012) / S- / locus early flowering 3 is present in the genomes of short-styled buckwheat plants that exhibit heteromorphic self-incompatibility. PLoS ONE 7(2):e31264-1鈥揺31264-9 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Plants in the genus Turnera provide a useful system for investigating the molecular genetics of distyly and candidate genes involved in this plant breeding system have been proposed. We develop the first transformation system for the genus, using leaf explant tissue of Turnera joelii transformed using Agrobacterium tumefaciens harbouring the pGreen plasmid. Transgenic plants were successfully regenerated using a two growth-medium method and hygromycin resistance as the selectable marker. This should provide a system for testing the function of candidate genes for distyly. We obtained 19 transgenic plants and explore some of their characteristics. We find at least two instances where chromosomal abnormalities have occurred during the transformation process and observe reduced pollen fertility for seven of the transformants, likely evidence for somaclonal variation. We show that the transgenes inserted into two transgenic plants exhibit expected single gene segregation ratios, but find aberrant ratios for a third transgenic plant. The latter plant exhibits a mutant self-compatible phenotype, where self-compatibility is due to a defect in the incompatibility of its pollen. In crosses, the self-compatible transgenic plant shows marked departures from expected single locus ratios at the S-locus determining distyly, with an almost complete lack of transmission of the dominant S-allele. The plant appears to contain more than one copy of the inserted transgene based upon segregation ratios.