Identification and characterization of plant-specific NAC gene family in canola (Brassica napus L.) reveal novel members involved in cell death
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  • 作者：Boya Wang (1)
    Xiaohua Guo (1)
    Chen Wang (1)
    Jieyu Ma (1)
    Fangfang Niu (1)
    Hanfeng Zhang (1)
    Bo Yang (1)
    Wanwan Liang (1)
    Feng Han (1)
    Yuan-Qing Jiang (1)

    1. State Key Laboratory of Crop Stress Biology for Arid Areas     ; and College of Life Sciences ; Northwest A & F University ; Yangling ; 712100 ; Shaanxi ; China
  • 关键词：Abiotic stress ; Brassica napus ; Cell death ; NAC ; ROS ; Transcription factors
  • 刊名：Plant Molecular Biology
  • 出版年：2015
  • 出版时间：March 2015
  • 年：2015
  • 卷：87
  • 期：4-5
  • 页码：395-411
  • 全文大小：2,205 KB
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  • 刊物类别：Biomedical and Life Sciences
  • 刊物主题：Life Sciences
    Plant Sciences
    Biochemistry
    Plant Pathology
  • 出版者：Springer Netherlands
  • ISSN：1573-5028
文摘
NAC transcription factors are plant-specific and play important roles in plant development processes, response to biotic and abiotic cues and hormone signaling. However, to date, little is known about the NAC genes in canola (or oilseed rape, Brassica napus L.). In this study, a total of 60 NAC genes were identified from canola through a systematical analysis and mining of expressed sequence tags. Among these, the cDNA sequences of 41 NAC genes were successfully cloned. The translated protein sequences of canola NAC genes with the NAC genes from representative species were phylogenetically clustered into three major groups and multiple subgroups. The transcriptional activities of these BnaNAC proteins were assayed in yeast. In addition, by quantitative real-time RT-PCR, we further observed that some of these BnaNACs were regulated by different hormone stimuli or abiotic stresses. Interestingly, we successfully identified two novel BnaNACs, BnaNAC19 and BnaNAC82, which could elicit hypersensitive response-like cell death when expressed in Nicotiana benthamiana leaves, which was mediated by accumulation of reactive oxygen species. Overall, our work has laid a solid foundation for further characterization of this important NAC gene family in canola.

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