Expression of the TRPM6 in mouse placental trophoblasts; potential role in maternal–fetal calcium transport

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• 作者：Yoshiro Suzuki ; Masaki Watanabe ; Claire T. Saito…
• 关键词：TRPM6 ; TRPM7 ; Maternal–fetal calcium transport ; Placenta ; Trophoblasts
• 刊名：The Journal of Physiological Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：67
• 期：1
• 页码：151-162
• 全文大小：
• 刊物主题：Human Physiology; Neurosciences; Animal Biochemistry; Animal Physiology; Cell Physiology; Neurobiology;
• 出版者：Springer Japan
• ISSN：1880-6562
• 卷排序：67

文摘

The placenta is required to transport calcium (Ca2+) from mother to fetus during fetal bone mineralization. In an attempt to clarify the molecular basis of Ca2+ entry for this transport, we identified TRPM6 as a candidate for apical Ca2+ entry pathway. TRPM6 mRNA increased during the last 4 days of pregnancy, coinciding with fetal bone mineralization in mice. TRPM6 mRNA and protein was localized in the trophoblasts in labyrinth where the maternal–fetal Ca2+ transport occurs. In patch-clamp recordings, we observed TRPM6/TRPM7-like currents in mouse trophoblasts after starting fetal bone mineralization but not before mineralization. Plasma membrane Ca2+ permeability was significantly increased in TRPM6/TRPM7 expressed HEK293 cells under physiological Mg2+ and ATP concentration but not in TRPM6 or TRPM7 homomer-expressing cells. These results suggest that TRPM6 is functionally expressed in mouse placental trophoblasts, implicating in maternal–fetal Ca2+ transport likely with TRPM7, which might enable to sustain fetal bone mineralization.