An immunoassay for the detection of triclosan-O-glucuronide, a primary human urinary metabolite of triclosan

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• 作者：Anupama Ranganathan ; Shirley J. Gee…
• 关键词：Triclosan ; O ; glucuronide ; Urinary metabolite ; Biomarker ; Polyclonal antibodies ; ELISA
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：407
• 期：24
• 页码：7263-7273
• 全文大小：872 KB
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• 作者单位：Anupama Ranganathan (1)
  Shirley J. Gee (1)
  Bruce D. Hammock (1)
  
  1. Department of Entomology and UCD Comprehensive Cancer Center, University of California Davis, Davis, CA, 95616, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Triclosan-O-glucuronide (TCSG) is one of the primary urinary metabolites of the antibacterial compound triclosan or TCS that is found in many personal care products and consumer goods. We have developed a competitive, indirect heterologous ELISA for the detection of the target TCSG in urine. Such an ELISA for TCSG could be developed as a useful tool to measure this important biomarker of human exposure to TCS. Immunogens were prepared by conjugating TCSG to thyroglobulin, via heterobifunctional cross-linkers AEDP or 3-[(2-aminoethyl)dithio] propionic acid?hydrochloride and TFCS or N-[ε-trifluoroacetylcaproyloxy]succinimide ester. The coating antigen was prepared by the direct conjugation of TCSG to bovine serum albumin. Antibodies raised in rabbits 2619, 2621 (immunogen TCSG-AEDP-Thy), and 2623 (immunogen TCSG-TFCS-Thy), and the coating antigen were screened and characterized to determine their optimal concentrations. The optimized ELISA, developed with antibody 2621, gave an IC₅₀ value of 2.85 ng/mL, with the linear range (IC₂₀–IC₈₀) determined to be 2.6-4.8 ng/mL. Selectivity of the assay was assessed by measuring cross-reactivity of antibody 2621 to related congeners such as the aglycone TCS, triclosan-O-sulfate, triclocarban, a polybrominated diphenyl ether derivative, and 3-phenoxybenzyl alcohol glucuronide. There was virtually no recognition by antibody 2621 to any of these cross-reactants.