Formation of GABA_A receptor complexes containing α1 and α5 subunits is paralleling a multiple T-maze learning task in mice

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• 作者：Maryam Ghafari ; Soheil Keihan Falsafi ; Edit Szodorai…
• 关键词：GABAA receptor complexes ; GABAAR α1 KO mice ; Multiple T ; maze ; Spatial memory ; BN ; PAGE ; Mass spectrometry
• 刊名：Brain Structure and Function
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：222
• 期：1
• 页码：549-561
• 全文大小：
• 刊物主题：Neurosciences; Cell Biology; Neurology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1863-2661
• 卷排序：222

文摘

There is limited information on the role of GABA type A receptors (GABA_ARs) containing α1, α5 and γ2 subunits in learning and memory. Here, we assessed the possible role of such receptors in spatial learning using the multiple T-maze (MTM) paradigm. C57BL/6J mice were trained in the MTM which induced elevated levels of α1 and α5 subunit-containing hippocampal GABA_AR complexes. Moreover, spatial learning evoked a significant increase in the colocalization of α1 and α5 subunits in both, CA1 and dentate gyrus regions of the hippocampus suggesting the formation of complexes containing both subunits. Additionally, the presence of α1, α5 and γ2 subunits in high molecular weight GABA_ARs was detected and significant correlation in the level of α1-containing complexes with those containing α5 and γ2 subunits was demonstrated. Accordingly, α1 deficiency led to decreased levels of γ2 subunit-containing complexes, however, had no effect on α5-containing ones. On the other hand, α1 knockout mice showed impaired performance in the MTM correlating with increased levels of α5 subunit-containing GABA_ARs in comparison to trained floxed control animals which quickly learned the task. Taken together, these results suggest that α1, α5 and γ2-containing hippocampal GABA_AR complexes play an essential role in spatial learning and memory in which targeted disruption of the α1 subunit produces profound deficits.