Probing the Microenvironment of Mammary Tumors Using Multiphoton Microscopy

详细信息    查看全文

• 作者：Mazen Sidani ; Jeffrey Wyckoff ; Chengsen Xue ; Jeffrey E. Segall and John Condeelis
• 关键词：Intravital imaging ; GFP ; Real ; time imaging ; Breast cancer ; Second harmonic generation
• 刊名：Journal of Mammary Gland Biology and Neoplasia
• 出版年：2006
• 出版时间：April, 2006
• 年：2006
• 卷：11
• 期：2
• 页码：151-163
• 全文大小：786 KB

文摘

Advances in optical imaging technologies that allow the subcellular resolution of undissected tissue have begun to offer new clues into the biology of development and disease. For cancer, such advances mean that the primary tumor is no longer a black box and that the disease can be studied throughout the metastatic cascade and not just as an endpoint. In this review we examine the advances in multiphoton imaging technology that have been used to define the microenvironment and its role in delineating the invasion and intravasation steps of metastasis inside living mammary tumors. Results show that the tumor microenvironment is a dynamic place where interactions between tumor cells, macrophages, blood vessels, and extracellular matrix fibers define the metastatic phenotype.