High prevalence and two dominant host-specific genotypes of Coxiella burnetii in U.S. milk
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- 作者：Talima Pearson (1)
Heidie M Hornstra (1)
Remy Hilsabeck (1)
Lauren T Gates (1)
Sonora M Olivas (1)
Dawn M Birdsell (1)
Carina M Hall (1)
Sabrina German (1)
James M Cook (1)
Meagan L Seymour (1)
Rachael A Priestley (2)
Ashley V Kondas (2)
Christine L Clark Friedman (1)
Erin P Price (1) (4)
James M Schupp (3)
Cindy M Liu (1) (3)
Lance B Price (3)
Robert F Massung (2)
Gilbert J Kersh (2)
Paul Keim (1) (3) - 关键词：Coxiella burnetii ; Q fever ; Environmental detection ; Genotyping ; Phylogeography ; Multispacer typing ; SNP typing ; Canonical SNP ; CanSNP
- 刊名：BMC Microbiology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：440 KB
- 参考文献：1. Karagiannis I, Schimmer B, Van Lier A, Timen A, Schneeberger P, Van Rotterdam B, Be Bruin A, Wijkmans C, Rietveld A, Van Duynhoven Y: Investigation of a Q fever outbreak in a rural area of The Netherlands. / Epidemiol Infect 2009,137(9):1283鈥?294. CrossRef
2. Tissot-Dupont H, Amadei MA, Nezri M, Raoult D: Wind in November, Q fever in December. / Emerg Infect Dis 2004,10(7):1264鈥?269. CrossRef
3. Benenson AS, Tigertt WD: Studies on Q fever in man. / Trans Assoc Am Phys 1956, 69:98鈥?04.
4. Agerholm J: Coxiella burnetii associated reproductive disorders in domestic animals-a critical review. / Acta Vet Scand 2013,55(1):13. CrossRef
5. Guatteo R, Seegers H, Taurel A-F, Joly A, Beaudeau F: Prevalence of Coxiella burnetii infection in domestic ruminants: a critical review. / Vet Microbiol 2011,149(1鈥?):1鈥?6. CrossRef
6. Astobiza I, Ruiz-Fons F, Pinero A, Barandika JF, Hurtado A, Garcia-Perez AL: Estimation of Coxiella burnetii prevalence in dairy cattle in intensive systems by serological and molecular analyses of bulk-tank milk samples. / J Dairy Sci 2012,95(4):1632鈥?638. CrossRef
7. Banazis MJ, Bestall AS, Reid SA, Fenwick SG: A survey of Western Australian sheep, cattle and kangaroos to determine the prevalence of Coxiella burnetii . / Vet Microbiol 2010,143(2鈥?):337鈥?45. CrossRef
8. Gyuranecz M, Denes B, Hornok S, Kovacs P, Horvath G, Jurkovich V, Varga T, Hajtos I, Szabo R, Magyar T, / et al.: Prevalence of Coxiella burnetii in Hungary: screening of dairy cows, sheep, commercial milk samples, and ticks. / Vector Borne Zoonotic Dis (Larchmont, NY) 2012,12(8):650鈥?53. CrossRef
9. Jones RM, Twomey DF, Hannon S, Errington J, Pritchard GC, Sawyer J: Detection of Coxiella burnetii in placenta and abortion samples from British ruminants using real-time PCR. / Vet Rec 2010, 167:965鈥?67. CrossRef
10. Rahimi E, Doosti A, Ameri M, Kabiri E, Sharifian B: Detection of Coxiella burnetii by Nested PCR in Bulk Milk Samples from Dairy Bovine, Ovine, and Caprine Herds in Iran. / Zoonoses Public Health 2009,57(7鈥?):e38-e41. CrossRef
11. Eldin C, Angelakis E, Renvois茅 A, Raoult D: Coxiella burnetii DNA, but not viable bacteria, in dairy products in France. / AmJTrop Med Hyg 2013,88(4):765鈥?69. CrossRef
12. Tilburg JJHC, Roest HJIJ, Nabuurs-Franssen MH, Horrevorts AM, Klaassen CHW: Genotyping reveals the presence of a predominant genotype of Coxiella burnetii in consumer milk products. / J Clin Microbiol 2012,50(6):2156鈥?158. CrossRef
13. Kim SG, Kim EH, Lafferty CJ, Dubovi E: Coxiella burnetii in bulk tank milk samples: United States. / Emerg Infect Dis 2005,11(4):619鈥?21. CrossRef
14. Kersh GJ, Wolfe TM, Fitzpatrick KA, Candee AJ, Oliver LD, Patterson NE, Self JS, Priestley RA, Loftis AD, Massung RF: Presence of Coxiella burnetii DNA in the environment of the United States (2006鈥?008). / Appl Environ Microbiol 2010, 76:4469鈥?475. CrossRef
15. de Bruin A, Janse I, Koning M, de Heer L, van der Plaats RQJ, van Leuken JPG, van Rotterdam BJ: Detection of Coxiella burnetii DNA in the environment during and after a large Q fever epidemic in the Netherlands. / J Appl Microbiol 2013,114(5):1395鈥?404. CrossRef
16. Hendrix LR, Samuel JE, Mallavia LP: Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE. / J Gen Microbiol 1991,137(2):269鈥?76. CrossRef
17. Arricau-Bouvery N, Hauck Y, Bejaoui A, Frangoulidis D, Bodier CC, Souriau A, Meyer H, Neubauer H, Rodolakis A, Vergnaud G: Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing. / BMC Microbiol 2006, 6:38. CrossRef
18. Svraka S, Toman R, Skultety L, Slaba K, Homan WL: Establishment of a genotyping scheme for Coxiella burnetii . / FEMS Microbiol Lett 2006,254(2):268鈥?74. CrossRef
19. Glazunova O, Roux V, Freylikman O, Sekeyova Z, Fournous G, Tyczka J, Tokarevich N, Kovacava E, Marrie TJ, Raoult D: Coxiella burnetii genotyping. / Emerg Infect Dis 2005,11(8):1211鈥?217.
20. Hornstra HM, Priestley RA, Georgia SM, Kachur S, Birdsell DN, Hilsabeck R, Gates LT, Samuel JE, Heinzen RA, Kersh GJ, / et al.: Rapid typing of Coxiella burnetii . / PLoS One 2011,6(11):e26201. CrossRef
21. Huijsmans CJ, Schellekens JJ, Wever PC, Toman R, Savelkoul PH, Janse I, Hermans MH: Single-nucleotide-polymorphism genotyping of Coxiella burnetii during a Q fever outbreak in The Netherlands. / Appl Environ Microbiol 2011,77(6):2051鈥?057. CrossRef
22. Pearson T, Hornstra HM, Sahl JW, Schaack S, Schupp JM, Beckstrom-Sternberg SM, O鈥橬eill MW, Priestley RA, Champion MD, Beckstrom-Sternberg JS, / et al.: When outgroups fail; phylogenomics of rooting the emerging pathogen, Coxiella burnetii . / Syst Biol 2013,62(5):752鈥?62. CrossRef
23. Keim P, Van Ert MN, Pearson T, Vogler AJ, Huynh LY, Wagner DM: Anthrax molecular epidemiology and forensics: using the appropriate marker for different evolutionary scales. / Infect Genet Evol 2004,4(3):205鈥?13. CrossRef
24. Van Ert MN, Easterday WR, Simonson TS, U鈥橰en JM, Pearson T, Kenefic LJ, Busch JD, Huynh LY, Dukerich M, Trim CB, / et al.: Strain-specific single-nucleotide polymorphism assays for the Bacillus anthracis Ames strain. / J Clin Microbiol 2007,45(1):47鈥?3. CrossRef
25. Price EP, Dale JL, Cook JM, Sarovich DS, Seymour ML, Ginther JL, Kaufman EL, Beckstrom-Sternberg SM, Mayo M, Kaestli M, / et al.: Development and validation of Burkholderia pseudomallei -specific real-time PCR assays for clinical, environmental or forensic detection applications. / PLoS ONE 2012,7(5):e37723. CrossRef
26. Loftis AD, Reeves WK, Szumlas DE, Abbassy MM, Helmy IM, Moriarity JR, Dasch GA: Rickettsial agents in Egyptian ticks collected from domestic animals. / Exp Appl Acarol 2006,40(1):67鈥?1. CrossRef
27. Astobiza I, Tilburg J, Pinero A, Hurtado A, Garcia-Perez A, Nabuurs-Franssen M, Klaassen C: Genotyping of Coxiella burnetii from domestic ruminants in northern Spain. / BMC Vet Res 2012,8(1):241. CrossRef
28. Tilburg JJHC, Roest H-JIJ, Buffet S, Nabuurs-Franssen MH, Horrevorts AM, Raoult D, Klaassen CHW: Epidemic genotype of Coxiella burnetii among goats, sheep, and humans in the Netherlands. / Emerg Infect Dis 2012,18(5):887鈥?89. CrossRef
29. Reichel R, Mearns R, Brunton L, Jones R, Horigan M, Vipond R, Vincent G, Evans S: Description of a Coxiella burnetii abortion outbreak in a dairy goat herd, and associated serology, PCR and genotyping results. / Res Vet Sci 2012,93(3):1217鈥?224. CrossRef
30. Santos AS, Tilburg JJHC, Botelho A, Barahona MJ, N煤ncio MS, Nabuurs-Franssen MH, Klaassen CHW: Genotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA typing. / Int J Med Microbiol 2012,302(6):253鈥?56. CrossRef
31. Kersh GJ, Fitzpatrick KA, Self JS, Priestley RA, Kelly AJ, Lash RR, Marsden-Haug N, Nett RJ, Bjork A, Massung RF, / et al.: Presence and persistence of Coxiella burnetii in the environments of goat farms associated with a Q fever outbreak. / Appl Environ Microbiol 2013,79(5):1697鈥?703. CrossRef
32. Chmielewski T, Sidi-Boumedine K, Duquesne V, Podsiadly E, Thiery R, Tylewska-Wierzbanowska S: Molecular epidemiology of Q fever in Poland. / Pol J Microbiol 2009,58(1):9鈥?3.
33. Tilburg JJHC, Rossen JWA, van Hannen EJ, Melchers WJG, Hermans MHA, van de Bovenkamp J, Roest HJIJ, de Bruin A, Nabuurs-Franssen MH, Horrevorts AM, / et al.: Genotypic diversity of Coxiella burnetii in the 2007鈥?010 Q fever outbreak episodes in The Netherlands. / J Clin Microbiol 2012,50(3):1076鈥?078. CrossRef
34. Garcia-Perez AL, Astobiza I, Barandika JF, Atxaerandio R, Hurtado A, Juste RA: Short communication: investigation of Coxiella burnetii occurrence in dairy sheep flocks by bulk-tank milk analysis and antibody level determination. / J Dairy Sci 2009,92(4):1581鈥?584. CrossRef
35. Schimmer B, Luttikholt S, Hautvast JLA, Graat EAM, Vellema P, Duynhoven YTHPV: Seroprevalence and risk factors of Q fever in goats on commercial dairy goat farms in the Netherlands, 2009鈥?010. / BMC Vet Res 2011, 7:81. CrossRef
36. McQuiston JH, Nargund VN, Miller JD, Priestley R, Shaw EI, Thompson HA: Prevalence of antibodies to Coxiella burnetii among veterinary school dairy herds in the United States, 2003. / Vector Borne Zoonotic Dis 2005,5(1):90鈥?1. CrossRef
37. Luoto L: Report on the nationwide occurrence of Q fever infections in cattle. / Pub Health Rep 1960, 75:135鈥?40. CrossRef
38. Milk cows and production by State and region (Annual) [http://www.ers.usda.gov/data-products/dairy-data.aspx#.UnwQGY3N-6I]
39. Rodolakis A, Berri M, Hechard C, Caudron C, Souriau A, Bodier CC, Blanchard B, Camuset P, Devillechaise P, Natorp JC, / et al.: Comparison of Coxiella burnetii shedding in milk of dairy bovine, caprine, and ovine herds. / J Dairy Sci 2007,90(12):5352鈥?360. CrossRef
40. Cabassi CS, Taddei S, Donofrio G, Ghidini F, Piancastelli C, Flammini CF, Cavirani S: Association between Coxiella burnetii seropositivity and abortion in dairy cattle of Northern Italy. / New Microbiol 2006,29(3):211鈥?14.
41. Langley JM, I: the disease: Perinatal Q fever: is Coxiella burnetii a human perinatal pathogen? In / Q fever. I: the disease edition. Edited by: Marrie TJ. Boca Raton, FL: CRC Press; 1990:201鈥?12.
42. Roest HJ, van Gelderen B, Dinkla A, Frangoulidis D, van Zijderveld F, Rebel J, van Keulen L: Q fever in pregnant goats: pathogenesis and excretion of Coxiella burnetii . / PLoS One 2012,7(11):e48949. CrossRef
43. Roest HIJ, Tilburg JJHC, van der Hoek W, Velleme P, Van Zijderveld FG, Klaassen CHW, Raoult D: The Q fever epidemic in The Netherlands: history, onset, response and reflection. / Epidemiol Infec 2011,139(01):1鈥?2. CrossRef
44. Tylewska-Wierzbanowska S, Kruszewska D, Chmielewski T: Epidemics of Q fever in Poland in 1992鈥?994. / Rocz Akad Med Bialymst 1996,41(1):123鈥?28.
45. Liu CM, Aziz M, Kachur S, Hsueh PR, Huang YT, Keim P, Price LB: BactQuant: an enhanced broad-coverage bacterial quantitative real-time PCR assay. / BMC Microbiol 2012, 12:56. CrossRef - 作者单位：Talima Pearson (1)
Heidie M Hornstra (1)
Remy Hilsabeck (1)
Lauren T Gates (1)
Sonora M Olivas (1)
Dawn M Birdsell (1)
Carina M Hall (1)
Sabrina German (1)
James M Cook (1)
Meagan L Seymour (1)
Rachael A Priestley (2)
Ashley V Kondas (2)
Christine L Clark Friedman (1)
Erin P Price (1) (4)
James M Schupp (3)
Cindy M Liu (1) (3)
Lance B Price (3)
Robert F Massung (2)
Gilbert J Kersh (2)
Paul Keim (1) (3)
1. Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ, 86011-4073, USA
2. Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA
4. Global and Tropical Health Division, Menzies School of Health Research, Tiwi, Northern Territory, 0810, Australia
3. Pathogen Genomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA - ISSN：1471-2180
文摘
Background Coxiella burnetii causes Q fever in humans and Coxiellosis in animals; symptoms range from general malaise to fever, pneumonia, endocarditis and death. Livestock are a significant source of human infection as they shed C. burnetii cells in birth tissues, milk, urine and feces. Although prevalence of C. burnetii is high, few Q fever cases are reported in the U.S. and we have a limited understanding of their connectedness due to difficulties in genotyping. Here, we develop canonical SNP genotyping assays to evaluate spatial and temporal relationships among C. burnetii environmental samples and compare them across studies. Given the genotypic diversity of historical collections, we hypothesized that the current enzootic of Coxiellosis is caused by multiple circulating genotypes. We collected A) 23 milk samples from a single bovine herd, B) 134 commercial bovine and caprine milk samples from across the U.S., and C) 400 bovine and caprine samples from six milk processing plants over three years. Results We detected C. burnetii DNA in 96% of samples with no variance over time. We genotyped 88.5% of positive samples; bovine milk contained only a single genotype (ST20) and caprine milk was dominated by a second type (mostly ST8). Conclusions The high prevalence and lack of genotypic diversity is consistent with a model of rapid spread and persistence. The segregation of genotypes between host species is indicative of species-specific adaptations or dissemination barriers and may offer insights into the relative lack of human cases and characterizing genotypes.