Fluorescence-based monitoring of ribosome assembly landscapes
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- 作者：Rainer Nikolay (1) (3)
Renate Schloemer (1)
Silke Mueller (2)
Elke Deuerling (1)
1. Molecular Microbiology ; University of Konstanz ; Constance ; 78457 ; Germany
3. Current address ; Institute of Medical Physics and Biophysics ; Charit茅-Universitaetsmedizin Berlin ; Berlin ; 10117 ; Germany
2. Screening Center Konstanz ; University of Konstanz ; Constance ; 78457 ; Germany - 关键词：Ribosome assembly ; Ribosome biogenesis ; Fluorescent proteins ; Antimicrobials ; Knock out ; 位 ; red recombineering ; High throughput screening
- 刊名：BMC Molecular Biology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：16
- 期：1
- 全文大小：2,137 KB
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- 出版者：BioMed Central
- ISSN：1471-2199
文摘
Background Ribosomes and functional complexes of them have been analyzed at the atomic level. Far less is known about the dynamic assembly and degradation events that define the half-life of ribosomes and guarantee their quality control. Results We developed a system that allows visualization of intact ribosomal subunits and assembly intermediates (i.e. assembly landscapes) by convenient fluorescence-based analysis. To this end, we labeled the early assembly ribosomal proteins L1 and S15 with the fluorescent proteins mAzami green and mCherry, respectively, using chromosomal gene insertion. The reporter strain harbors fluorescently labeled ribosomal subunits that operate wild type-like, as shown by biochemical and growth assays. Using genetic and chemical perturbations by depleting genes encoding the ribosomal proteins L3 and S17, respectively, or using ribosome-targeting antibiotics, we provoked ribosomal subunit assembly defects. These defects were readily identified by fluorometric analysis after sucrose density centrifugation in unprecedented resolution. Conclusion This strategy is useful to monitor and characterize subunit specific assembly defects caused by ribosome-targeting drugs that are currently used and to characterize new molecules that affect ribosome assembly and thereby constitute new classes of antibacterial agents.