Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea
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  • 作者:Yan Li ; Min Wang ; Qian Liu ; Xue Song ; Duobing Wang ; Yu Ma…
  • 刊名:Current Microbiology
  • 出版年:2016
  • 出版时间:May 2016
  • 年:2016
  • 卷:72
  • 期:5
  • 页码:628-633
  • 全文大小:575 KB
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  • 作者单位:Yan Li (1)
    Min Wang (1)
    Qian Liu (1)
    Xue Song (1)
    Duobing Wang (1)
    Yu Ma (1)
    Hongbing Shao (1)
    Yong Jiang (1)

    1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China
  • 刊物类别:Biomedical and Life Sciences
  • 刊物主题:Life Sciences
    Microbiology
    Biotechnology
  • 出版者:Springer New York
  • ISSN:1432-0991
文摘
Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage titers is noted at temperature 30-50 °C. A latent period of approximately 96 mins is indicated by the one-step growth curve. And, the burst size is about three virions per cell. Furthermore, genomic analysis of H188 reveals a genome size of 50364 bp with 43.63 % G+C content, and 76 putative open reading frames. There is no obvious similarity between H188 and other known phages by genomic comparison. Moreover, the H188 genome includes modules for phage structure, phage packaging, DNA replication and regulation, and some additional functions.

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