Expression of Bmi1, FoxF1, Nanog, and γ-Catenin in Relation to Hedgehog Signaling Pathway in Human Non-small-Cell Lung Cancer

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• 作者：Ioannis P. Gialmanidis ; Vasiliki Bravou ; Ilias Petrou ; Helen Kourea…
• 关键词：Bmi1 ; FoxF1 ; Hedgehog ; Nanog ; NSCLC
• 刊名：Lung
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：191
• 期：5
• 页码：511-521
• 全文大小：866KB
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• 作者单位：Ioannis P. Gialmanidis (1)
  Vasiliki Bravou (1)
  Ilias Petrou (1)
  Helen Kourea (2)
  Alexandros Mathioudakis (3)
  Ioannis Lilis (1)
  Helen Papadaki (1)
  
  1. Department of Anatomy, School of Medicine, University of Patras, 26500, Rio Patras, Greece
  2. Department of Pathology, School of Medicine, University of Patras, 26500, Rio Patras, Greece
  3. Southport and Ormskirk Hospital NHS Trust, Southport, Merseyside, PR8 6PN, UK
  
• ISSN：1432-1750

文摘

Background Hedgehog signaling is known to be involved in both lung organogenesis and lung carcinogenesis. The aim of this study was to examine potential downstream targets of the hedgehog signaling pathway in non-small-cell lung cancer. Methods Protein expression of Bmi1, FoxF1, Nanog, and γ-catenin was examined by immunohistochemistry in 80 non-small-cell lung cancer samples. Correlations with the previously immunohistochemically recovered results for sonic hedgehog, Ptch1, Smo, Gli1, and Gli2 in the same cohort of tumors as well as the clinicopathological characteristics of the tumors were also evaluated. Results Bmi1 was expressed in 78/80 (97.5?%) cases of non-small-cell lung cancer and correlated with male gender and expression of Gli1. Positive expression of FoxF1 was found in 62/80 (77.5?%) cases. Expression of FoxF1 correlated with lymph node metastases, Bmi1, and hedgehog pathway activation. Overexpression of Nanog was also noted in 74/80 (92.5?%) tumors and correlated with Bmi1. Cytoplasmic accumulation of γ-catenin was observed in 85?% (68/80) of the tumors and correlated with the expression of Bmi1, FoxF1, and Nanog. Conclusion Several developmental pathways seem to be implicated in non-small-cell lung cancer. It is also suggested that Bmi1 and FoxF1 may cooperate with hedgehog signaling in non-small-cell lung carcinogenesis.