Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models

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• 作者：Matteo Cornaglia ; Gopalan Krishnamani ; Laurent Mouchiroud…
• 关键词：Caenorhabditis elegans ; Neurodegenerative disease ; Amyotrophic lateral sclerosis (ALS) ; Huntington disease (HD) ; Doxycycline treatment ; Protein aggregation ; Longitudinal time ; resolved analysis ; High ; resolution imaging ; Worm immobilization ; Temperature control ; Microfluidics
• 刊名：Molecular Neurodegeneration
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：11
• 期：1
• 全文大小：3,247 KB
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• 作者单位：Matteo Cornaglia (1)
  Gopalan Krishnamani (1)
  Laurent Mouchiroud (2)
  Vincenzo Sorrentino (2)
  Thomas Lehnert (1)
  Johan Auwerx (2)
  Martin A. M. Gijs (1)
  
  1. Laboratory of Microsystems, EPFL, CH-1015, Lausanne, Switzerland
  2. Laboratory for Integrative and Systems Physiology, EPFL, CH-1015, Lausanne, Switzerland
  
• 刊物主题：Neurosciences; Neurology; Molecular Medicine;
• 出版者：BioMed Central
• ISSN：1750-1326

文摘

Background While many biological studies can be performed on cell-based systems, the investigation of molecular pathways related to complex human dysfunctions – e.g. neurodegenerative diseases – often requires long-term studies in animal models. The nematode Caenorhabditis elegans represents one of the best model organisms for many of these tests and, therefore, versatile and automated systems for accurate time-resolved analyses on C. elegans are becoming highly desirable tools in the field.